PRIMER_EXPLAIN_FLAG=1
PRIMER_PRODUCT_SIZE_RANGE=36-2000
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_GC_CLAMP=0
PRIMER_OPT_SIZE=20
PRIMER_MIN_SIZE=18
PRIMER_MAX_SIZE=27
PRIMER_OPT_TM=60.0
PRIMER_MIN_TM=57.0
PRIMER_MAX_TM=63.0
PRIMER_PAIR_MAX_DIFF_TM=100.0
PRIMER_MIN_GC=20.0
PRIMER_MAX_GC=80.0
PRIMER_SALT_MONOVALENT=50.0
PRIMER_DNA_CONC=50.0
PRIMER_MAX_NS_ACCEPTED=0
PRIMER_MAX_SELF_ANY=8.00
PRIMER_MAX_SELF_END=3.00
PRIMER_PAIR_MAX_COMPL_ANY=8.00
PRIMER_PAIR_MAX_COMPL_END=3.00
PRIMER_MAX_POLY_X=5
PRIMER_LIBERAL_BASE=0
PRIMER_NUM_RETURN=1
PRIMER_FIRST_BASE_INDEX=0
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
PRIMER_MAX_LIBRARY_MISPRIMING=11.00
PRIMER_OUTSIDE_PENALTY=20.0
PRIMER_INSIDE_PENALTY=1000.0
SEQUENCE_ID=AT3_X1
SEQUENCE_TARGET=298,45
SEQUENCE_TEMPLATE=TTCTATAAAGCTGAGGAGAAGAGTGAGGGAGTGTGGGCAAGAGAGGTGGCTCAGGCTTTCCCTGGGCCTGATTGAACTTTAAAACTTCTCTACTAATTAAACAACACTGGGCTCTACACTTTGCTTAACCCTGGGAACTGGTCATCAGCCTTTGACCTCAGTTCCCCCTCCTGACCAGCTCTCTGCCCCACCCTGTCCTCTGGAACCTCTGCGAGATTTAGAGGAAAGAACCAGTTTTCAGGCGGATTGCCTCAGATCACACTATCTCCACTTGCCCAGCCCTGTGGAAGATTAGCGGCCATGTATTCCAATGTGATAGGAACTGTAACCTCTGGAAAAAGGTAAGAGGGGTGAGCTTTCCCCTTGCCTGCCCCTACTGGGTTTTGTGACCTCCAAAGGACTCACAGGAATGACCTCCAACACCTTTGAGAAGACCAGGCCCTCTCCCTGGTAGTTACAGTCAAAGACCTGTTTGGAAGACGTCATTTCAAGTGCTCTCCCTCCCACCCCACCTCTTGGGGTAAGGCCTTTCCTAAGCTACCCCTTGGGTCCCTAGCCTAAGAAACAAGGGGGATGTCATCCCTGGTGTAAAGATGCTGTGCAGGAAGTCAGCACTCACGGGATCCAGGGGACGCTCCAAG
PRIMER_LEFT_EXPLAIN=considered 3504, overlap target 289, GC content failed 16, low tm 818, high tm 1648, ok 733
PRIMER_RIGHT_EXPLAIN=considered 3504, overlap target 289, low tm 655, high tm 1723, ok 837
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.180022
PRIMER_LEFT_0_PENALTY=1.599465
PRIMER_RIGHT_0_PENALTY=1.580558
PRIMER_LEFT_0_SEQUENCE=CCCTGTGGAAGATTAGCGG
PRIMER_RIGHT_0_SEQUENCE=GGGAAAGCTCACCCCTCTT
PRIMER_LEFT_0=279,19
PRIMER_RIGHT_0=361,19
PRIMER_LEFT_0_TM=60.599
PRIMER_RIGHT_0_TM=60.581
PRIMER_LEFT_0_GC_PERCENT=57.895
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=7.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=11.4000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=83
PRIMER_PAIR_0_PRODUCT_TM=72.4
=
SEQUENCE_ID=AT3_X2
SEQUENCE_TARGET=298,371
SEQUENCE_TEMPLATE=AAACAGGAAGCTTCTCATTCTTTTTTCTCCCAGCGTGGGGAATTGAGCACATAATACTCCAAATAACCATCAGATGATTCTAATTCCAACATGACCACGTCCAGGCAACTGAACTGTCCCCTGGCAAGAAGTCTAGGACTGAACCTGTCCCGGGCCCCTGTACTTGGTTCAAAGGATTTAGCCTTTCTCTTGGCCACACCAGGTGGGCTGGAATCCTCTGCTTTACTGGGGCAACCCTGTGGTGGGCAGTGGGGCTAGGGGTTGCAGCCTAGCTTAACTTGGCATTTTGTCTCCTTGCAGGAAGGTTTATCTTTTGTCCTTGCTGCTCATTGGCTTCTGGGACTGCGTGACCTGTCACGGGAGCCCTGTGGACATCTGCACAGCCAAGCCGCGGGACATTCCCATGAATCCCATGTGCATTTACCGCTCCCCGGAGAAGAAGGCAACTGAGGATGAGGGCTCAGAACAGAAGATCCCGGAGGCCACCAACCGGCGTGTCTGGGAACTGTCCAAGGCCAATTCCCGCTTTGCTACCACTTTCTATCAGCACCTGGCAGATTCCAAGAATGACAATGATAACATTTTCCTGTCACCCCTGAGTATCTCCACGGCTTTTGCTATGACCAAGCTGGGTGCCTGTAATGACACCCTCCAGCAACTGATGGAGGTACGACCAAAGGTCTTCTGCCCAGCCACCTTGTTAGGAGCACCTTTGGGCTTCCATAGGCCCAAGTCCAATGATTCCTCAACCAACACTGCAGCCACTAGGGGCGCTCATTATGCATTACGATTCCCTTTGAACATCACTGTGTTATAATTCCCTTTGAAAATCATTTTTTAAAAAATTAGCCAAGGAATCTTGGCTATCTACTTTTTAAATCCTGGTTTCCTCTTTTGAGCACCTTAAAATGGGGGAAGGCTTGTATCTTCTCTCAACTTCTTTTCAGTAATTCTTTCATCTATATGT
PRIMER_LEFT_EXPLAIN=considered 6612, overlap target 289, GC content failed 1, low tm 1035, high tm 3921, long poly-x seq 22, ok 1344
PRIMER_RIGHT_EXPLAIN=considered 6657, overlap target 289, GC content failed 114, low tm 1327, high tm 3439, high end compl 2, long poly-x seq 19, ok 1467
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=22.934112
PRIMER_LEFT_0_PENALTY=1.148919
PRIMER_RIGHT_0_PENALTY=21.785193
PRIMER_LEFT_0_SEQUENCE=TTGGCATTTTGTCTCCTTGC
PRIMER_RIGHT_0_SEQUENCE=GGCAGAAGACCTTTGGTCG
PRIMER_LEFT_0=278,20
PRIMER_RIGHT_0=688,19
PRIMER_LEFT_0_TM=61.149
PRIMER_RIGHT_0_TM=60.785
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, MIR2 Repetitive element - a consensus
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=20.000000
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=9.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=411
PRIMER_PAIR_0_PRODUCT_TM=80.3
=
SEQUENCE_ID=AT3_X3
SEQUENCE_TARGET=298,220
SEQUENCE_TEMPLATE=CAAGAGGACTTTTCCCATTCCAGCAAGAAAACGTCTGTGTGTTGATCTAGAGGCGTTTAGAGACTTTAGGTGGCAACCTAGTCTCTCTTTTTCCCTTTATCCTTCCTACCCTTCATTCTTCTTTTATCCTTTTATTCATCAGAACACAAGAGTTGAGCATTTATGCTGTCCCAGGTACTGTGCTTGAAGGAGTTAACAACTGAGGTGGCTATTAGTCAGAGACTGACCAGCATGTGCTCACCACCCATGTTAACTAGGCAGCCCACCAAACCCACCACCATTTTTTTTTGACTTCTATAGGTATTTAAGTTTGACACCATATCTGAGAAAACATCTGATCAGATCCACTTCTTCTTTGCCAAACTGAACTGCCGACTCTATCGAAAAGCCAACAAATCCTCCAAGTTAGTATCAGCCAATCGCCTTTTTGGAGACAAATCCCTTACCTTCAATGAGACCTACCAGGACATCAGTGAGTTGGTATATGGAGCCAAGCTCCAGCCCCTGGACTTCAAGGTGAGTTGCAGATGTTACCCCTGACCTCCGAGTTCTTCCTCTCCACTCAGAGATTGAGGAGGTGGAGAAACAGCATCCAAATTCACACTGCTTTGCTGCTGAAGACTGCTGGAGGGCTGACTAAAAGTTAGAACCCCTGCAATAGTTATTCTTACTTGAAACCTGAGAAATCAAAGGTATCCATGCTTGGATTGTAGTGACTGCCCAGAAAACATGAATTAATAATCAATTCTTCATTCCATCCACCAACTTCAAATATATACCAAAGGGTGTTTTGAAGATGCCAGTTCTACAAGATAT
PRIMER_LEFT_EXPLAIN=considered 5081, overlap target 289, GC content failed 13, low tm 1874, high tm 1352, long poly-x seq 25, ok 1528
PRIMER_RIGHT_EXPLAIN=considered 5254, overlap target 289, GC content failed 103, low tm 1706, high tm 1557, ok 1599
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=263.125741
PRIMER_LEFT_0_PENALTY=261.732281
PRIMER_RIGHT_0_PENALTY=1.393460
PRIMER_LEFT_0_SEQUENCE=CCAAACCCACCACCATTTTT
PRIMER_RIGHT_0_SEQUENCE=CAGGGGTAACATCTGCAACTC
PRIMER_LEFT_0=265,20
PRIMER_RIGHT_0=538,21
PRIMER_LEFT_0_TM=61.732
PRIMER_RIGHT_0_TM=59.607
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=260.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=274
PRIMER_PAIR_0_PRODUCT_TM=75.7
=
SEQUENCE_ID=AT3_X3'
SEQUENCE_TARGET=298,142
SEQUENCE_TEMPLATE=AGTCAGAGAAAATAATATTTTATCTTCTTTTTCCCAGACTATTTTCGCCTTCCTTCTTTTCATTTATTTCTTCCTATTTCTTTTTGTCTTTTTCTTCTGATAATATTTATTAACTACAGGAAAGATTCATGGAACTATATTAGATATGTGAGGCTTCCCAATTTGGGTTAGAGCAATGGCTTCTTAATCAAATGGTGGGAAAGGACAGAGGGATGGTGAGAAAAATAAAATGCTGCCTGGGAAAATGGAGAAGCCAATTGAATAGCACAGGTGAGTAGGTTTATTTTCTGTTCTCCTCAGGAAAATGCAGAGCAATCCAGAGCGGCCATCAACAAATGGGTGTCCAATAAGACCGAAGGCCGAATCACCGATGTCATTCCCTCGGAAGCCATCAATGAGCTCACTGTTCTGGTGCTGGTTAACACCATTTACTTCAAGGTACTCAGAATGGCCCTGGAGAGACCCCAGGGACTTCCTCTTGCTCTTCAGCTTACCCCCTTTTTTTTTAAATGGCGAGACCGAAGCCCTGAGAGGGCAAATGGACTGCCGAAAGCTACACAGGTACAGGTCAGCAGGGCAGGTCAATCTATTATTTATTTATTTATTTATTTTTGACAGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACTGCAAGCTTCGCCTCCTGGGTTCTCGGCATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGAATACAGGC
PRIMER_LEFT_EXPLAIN=considered 4474, overlap target 289, GC content failed 273, low tm 1464, high tm 1424, high repeat similarity 13, ok 1011
PRIMER_RIGHT_EXPLAIN=considered 4294, overlap target 289, GC content failed 209, low tm 512, high tm 2472, long poly-x seq 25, ok 787
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=128.448365
PRIMER_LEFT_0_PENALTY=127.241506
PRIMER_RIGHT_0_PENALTY=1.206859
PRIMER_LEFT_0_SEQUENCE=CACAGGTGAGTAGGTTTATTTTCTGTT
PRIMER_RIGHT_0_SEQUENCE=CTCCAGGGCCATTCTGAGT
PRIMER_LEFT_0=265,27
PRIMER_RIGHT_0=458,19
PRIMER_LEFT_0_TM=60.242
PRIMER_RIGHT_0_TM=60.207
PRIMER_LEFT_0_GC_PERCENT=37.037
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=120.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=194
PRIMER_PAIR_0_PRODUCT_TM=76.7
=
SEQUENCE_ID=AT3_X4
SEQUENCE_TARGET=298,395
SEQUENCE_TEMPLATE=GGCTTTGTCACTCATTTCTTCTTAGCATGGAATAGCATTACATTTGGTCTGGATGTACCACAGTCTGTCTATTCATCTACTGAAGGACATTTTGGCTGCTTCCAAGGTTTGACAGTTATGAATAAACCTACTCATAATTCCATCATTCTGACACAGCCATTGTTAACCTTTTTGTGCATATCCCGCCAGTCTTTTTTCCGAATAATTATATATTAATGTAACACTATAATATGGATATGTCTGTGTCAATAACTATCCTCCTATGAATGTTTGTGTTCTTACTTTGTGATTCTCTTCCAGGGCCTGTGGAAGTCAAAGTTCAGCCCTGAGAACACAAGGAAGGAACTGTTCTACAAGGCTGATGGAGAGTCGTGTTCAGCATCTATGATGTACCAGGAAGGCAAGTTCCGTTATCGGCGCGTGGCTGAAGGCACCCAGGTGCTTGAGTTGCCCTTCAAAGGTGATGACATCACCATGGTCCTCATCTTGCCCAAGCCTGAGAAGAGCCTGGCCAAGGTGGAGAAGGAACTCACCCCAGAGGTGCTGCAGGAGTGGCTGGATGAATTGGAGGAGATGATGCTGGTGGTCCACATGCCCCGCTTCCGCATTGAGGACGGCTTCAGTTTGAAGGAGCAGCTGCAAGACATGGGCCTTGTCGATCTGTTCAGCCCTGAAAAGTCCAAACTCCCAGGTTTGTCTAGGAAGGAGTTTCCTCCCTTCTCCACCCGCAAGGTAGTCTGACCAAAAGTGGAAGAGTTGGAGAAAGAATAGAAAGGAGCAACAAGTCAGGACTCCTGGATACTGATCCTAGTTTCTACTGCTAATTTGTGGAAATCTCTTTTCCTTTTGAGACCTCAGTTTCCTCTTCTGTAAAAGGGAAGTTTGTTCTTGGATCTCCATGGGCCCAGCCAGCACTGGTGCCCTGTGAGTCTGTATCAGGTAGAGGAGATGGGACCAGGTGGAGAGGAATTTGAAAGGGCATTGGAATTCA
PRIMER_LEFT_EXPLAIN=considered 6872, overlap target 289, GC content failed 226, low tm 1924, high tm 2932, long poly-x seq 17, ok 1484
PRIMER_RIGHT_EXPLAIN=considered 7004, overlap target 289, low tm 1493, high tm 3474, ok 1748
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=10.021418
PRIMER_LEFT_0_PENALTY=6.906112
PRIMER_RIGHT_0_PENALTY=3.115306
PRIMER_LEFT_0_SEQUENCE=TGTTCTTACTTTGTGATTCTCTTCC
PRIMER_RIGHT_0_SEQUENCE=GGAGGAAACTCCTTCCTAGACAA
PRIMER_LEFT_0=273,25
PRIMER_RIGHT_0=715,23
PRIMER_LEFT_0_TM=58.094
PRIMER_RIGHT_0_TM=60.115
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=47.826
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=443
PRIMER_PAIR_0_PRODUCT_TM=80.3
=
SEQUENCE_ID=AT3_X5
SEQUENCE_TARGET=298,69
SEQUENCE_TEMPLATE=TAGAGACAAGGTTTCACCATATTAGTTAGAGTGTCTCCAACTCCTGACCTCAGGTGATCTGTACACCTTGGCCTCCCGAAGTGCTGGGATTACAGGTGTGAGCCACTGCACCCAGCCCCCAAAGTACTTTATTATTTTTAACACATATTCATTGTGAGAGTATGATTAGGTGAAGATTTAGGATTTCTTCTTATGTTTCAAAAAGCCCCAAAGGATCTCTTAATCCAAACTGAATTCCCATCTGTGGATTGAAGCCAACTTTCTCCCATCTCACAAAGACTTCTCCGGTCTTCCTTCCAGGTATTGTTGCAGAAGGCCGAGATGACCTCTATGTCTCAGATGCATTCCATAAGGCATTTCTTGAGGTGAGTACACCTTCCCCACTCTCTTAGGGTACAGAAAGGAGATGCATGAACAGCAGGAACACGTGGAAAAGGCCTGTTTCCAGTGTTAAGGCATGCAAAAGGCCTCCACAGGCTGCTATAATACAGCCCTCTCCAAAACCTTCATGGTGTGATTGTTCTGCCTTCCCTCCCACTACCTCTTCTGTAGCAGGTCAAGCGGGAACACAAACATTTAGGGAGGGTGATATAGGAAAAGAAGCCAGCAAAGGCCATCAAGAAGAAATTTACAGCATGAGGAGAACCAGAAGAGTATGGGGTCGC
PRIMER_LEFT_EXPLAIN=considered 3744, overlap target 289, GC content failed 148, low tm 1249, high tm 1208, ok 850
PRIMER_RIGHT_EXPLAIN=considered 3744, overlap target 289, low tm 874, high tm 1504, high end compl 8, ok 1069
PRIMER_PAIR_EXPLAIN=considered 21, high end compl 20, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=45.783665
PRIMER_LEFT_0_PENALTY=22.061137
PRIMER_RIGHT_0_PENALTY=23.722528
PRIMER_LEFT_0_SEQUENCE=AGACTTCTCCGGTCTTCCTTC
PRIMER_RIGHT_0_SEQUENCE=AAGAGAGTGGGGAAGGTGTACT
PRIMER_LEFT_0=276,21
PRIMER_RIGHT_0=389,22
PRIMER_LEFT_0_TM=58.939
PRIMER_RIGHT_0_TM=58.277
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=20.000000
PRIMER_RIGHT_0_POSITION_PENALTY=20.000000
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=5.1000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=114
PRIMER_PAIR_0_PRODUCT_TM=74.4
=
SEQUENCE_ID=AT3_X6
SEQUENCE_TARGET=298,181
SEQUENCE_TEMPLATE=CCATTTACAATGGTACCAGCCAGAAATGTGATAACCTGTGTACATTCAGATTTCTGGGTTACCTGAATGGAACTCTTACACTTATTACCTAGCACAAGGCTTGGACAAACACAAGTACCTTACATTCTCTGCATGAAAGAATGAGTGAAAGTAGGATTCTGGAGGGAATCCAACCTGACCCAAATGTACTTTTTACTGGAAAACAAAAGCATTTGAGGAATTGCTGTGTCTGTGGATGATTTACCTGCCAAAATGAACGGCAGAGTGGCTAATTTAGTTTTATTCCCATGTGACCTGCAGGTAAATGAAGAAGGCAGTGAAGCAGCTGCAAGTACCGCTGTTGTGATTGCTGGCCGTTCGCTAAACCCCAACAGGGTGACTTTCAAGGCCAACAGGCCTTTCCTGGTTTTTATAAGAGAAGTTCCTCTGAACACTATTATCTTCATGGGCAGAGTAGCCAACCCTTGTGTTAAGTAAAATGTTCTTATTCTTTGCACCTCTTCCTATTTTTGGTTTGTGAACAGAAGTAAAAATAAATACAAACTACTTCCATCTCACATTATAAATGGACTCTGCATTTGAAATGAAGACAAGGAAAGGGGAAACATGCTATTGGGGCACATGGTAAAATGATGCCTTCAAGTTGTTCTTTACCCAGTAACCACATCTGGATCAAGAAAATGAGGGAGAGAGCGATAAAAGATGGTAGACAGCCAGAAAGGGAAGGGAGAG
PRIMER_LEFT_EXPLAIN=considered 4819, overlap target 244, low tm 1696, high tm 1512, ok 1367
PRIMER_RIGHT_EXPLAIN=considered 4414, overlap target 289, GC content failed 103, low tm 1578, high tm 1288, high repeat similarity 14, ok 1142
PRIMER_PAIR_EXPLAIN=considered 3, high end compl 2, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=65.942667
PRIMER_LEFT_0_PENALTY=1.577239
PRIMER_RIGHT_0_PENALTY=64.365428
PRIMER_LEFT_0_SEQUENCE=TTTATTCCCATGTGACCTGC
PRIMER_RIGHT_0_SEQUENCE=GGAAGAGGTGCAAAGAATAAGA
PRIMER_LEFT_0=278,20
PRIMER_RIGHT_0=503,22
PRIMER_LEFT_0_TM=58.423
PRIMER_RIGHT_0_TM=57.635
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=60.000000
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=6.0000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=226
PRIMER_PAIR_0_PRODUCT_TM=75.8
=
SEQUENCE_ID=CETP_X1
SEQUENCE_TARGET=298,122
SEQUENCE_TEMPLATE=AGTAAAAATAAATGAATTCATGGAAACATATTAAGCAATTATCCAGATAACATAAGGGATGGCAAAAATGGTGCAGATGGTGGAGGGGAGACAAGTAGAAGTTGGGGTGCTCTTGTTGAATGTCTGGCTCTGAACTCTAGAGGAGGCCGCAGGGGCTGGGCAGGAAGGAGGTGAATCTCTGGGGCCAGGAAGACCCTGCTGCCCGGAAGAGCCTCATGTTCCGTGGGGGCTGGGCGGACATACATATACGGGCTCCAGGCTGAACGGCTCGGGCCACTTACACACCACTGCCTGATAACCATGCTGGCTGCCACAGTCCTGACCCTGGCCCTGCTGGGCAATGCCCATGCCTGCTCCAAAGGCACCTCGCACGAGGCAGGCATCGTGTGCCGCATCACCAAGCCTGCCCTCCTGGTGTGTAAGTATCAGTGCATCTGTCTGCCCTGCCAGGGGTCTTTTCATGGACACCCACTATGCCAGGAGCCTCCCTGGCCTGAAGCCAGCCCTGAAGCCGGCTGCCACACTAGCCCAGAGAGAGGAGTGCCCTGGGAGGGAGATGGGCTGAGTGGAGCTGTCATCACCCCCTCCTGACCTCGCCTTCAAGGTCAAGTTCTTTGGTGAGAAGGTCCTAGCTGCATTGCAAACAGCCAGGTATAGGGATTTGTGTTTGTCTGCGACCCAGAATCACTGGGGTTCGAGTTAGGGTTCAGATCTGAGC
PRIMER_LEFT_EXPLAIN=considered 4274, overlap target 289, GC content failed 68, low tm 569, high tm 2779, ok 569
PRIMER_RIGHT_EXPLAIN=considered 4274, overlap target 289, low tm 375, high tm 2978, ok 632
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=7.862912
PRIMER_LEFT_0_PENALTY=4.158608
PRIMER_RIGHT_0_PENALTY=3.704304
PRIMER_LEFT_0_SEQUENCE=CTTACACACCACTGCCTGATAA
PRIMER_RIGHT_0_SEQUENCE=GGCAGACAGATGCACTGATACTT
PRIMER_LEFT_0=276,22
PRIMER_RIGHT_0=442,23
PRIMER_LEFT_0_TM=57.841
PRIMER_RIGHT_0_TM=60.704
PRIMER_LEFT_0_GC_PERCENT=45.455
PRIMER_RIGHT_0_GC_PERCENT=47.826
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=5.9000
PRIMER_RIGHT_0_END_STABILITY=5.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=167
PRIMER_PAIR_0_PRODUCT_TM=81.1
=
SEQUENCE_ID=CETP_X2
SEQUENCE_TARGET=298,119
SEQUENCE_TEMPLATE=CTAGGGTTAGTGTGCGGCTGGGCTCAGGGCTGCCCCATTTGCTAGGATCGTGGGGTTCCCATGTGTCAGGATCCAGAGGCTAGGGTATGATCAGGATCTCTAGCTGGGGTCAGGGTCAGAGCTCTCTGTGTCCCCTAGAATTGCCATCAACCTTAAACCCAGAGGAGGCCCAGTCCAACCCCTCAGCTTTAAGACCTGGGAGCCTCATCTCAGAGAGGCTGAGTCATGGCCAAGGCAGTTGGGGTGGGAGCAGGGGGCTTGGTGTGGGCCTGCAGCCCTCATCCACTGCCCTCCCTCTAGTGAACCACGAGACTGCCAAGGTGATCCAGACCGCCTTCCAGCGAGCCAGCTACCCAGATATCACGGGCGAGAAGGCCATGATGCTCCTTGGCCAAGTCAAGTATGGGTTGCACAAGTGAGTCGGGCCTCGGGTGTGACCTGGCTGGGGGTAGGGTGGCGGGAGGAACAGCCTGGGCTTCCCCCAGCCACAGGGAGGAAAGGCAGCAGCTGGGGGACTCAGGTCTCTCCCCTTGATTTGGAACCAGAGCC
PRIMER_LEFT_EXPLAIN=considered 4078, overlap target 123, GC content failed 7, low tm 374, high tm 2820, ok 754
PRIMER_RIGHT_EXPLAIN=considered 2584, overlap target 289, GC content failed 1, low tm 95, high tm 1901, ok 298
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=186.749017
PRIMER_LEFT_0_PENALTY=2.407131
PRIMER_RIGHT_0_PENALTY=184.341886
PRIMER_LEFT_0_SEQUENCE=ATCCACTGCCCTCCCTCT
PRIMER_RIGHT_0_SEQUENCE=AGCCAGGTCACACCCGAG
PRIMER_LEFT_0=280,18
PRIMER_RIGHT_0=443,18
PRIMER_LEFT_0_TM=59.593
PRIMER_RIGHT_0_TM=62.342
PRIMER_LEFT_0_GC_PERCENT=61.111
PRIMER_RIGHT_0_GC_PERCENT=66.667
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_PAIR_0_LIBRARY_MISPRIMING=15.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=180.000000
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=9.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=164
PRIMER_PAIR_0_PRODUCT_TM=80.5
=
SEQUENCE_ID=CETP_X3
SEQUENCE_TARGET=298,139
SEQUENCE_TEMPLATE=TGCCTCCTCTTCCACTTTTCGGTACCCTGTGATTGATTGGGACCACCCAGATAACCTAGGATCATCTCCCCACCTACCCCAAGGTCCTTAACTTAACCATACTTCATATGGGTAACACGAGTTGAGTGTGGTACCCAGGTTTGACATGTTGGGTAACATATTTGCAGGTTCTGTGGATTAGGAGGACATTTTGGGGGCCATGATTCTATCTTCCACCCTCGCCTAGACAAAATTGGAGGCTCACTCCTTGGGCTCCCTGGATGACCCCCAACATCCTTCCTCACTTCCATTCCTTCCCAGCATCCAGATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGAGCTGGTGGAAGCCAAGTCCATTGATGTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGGGACCCTGAAGTATGGCTACACCACTGCCTGGTGGTAAGCATTCCTGTCAGCTGATGCCCCATGCCCTGGCCCTCTCTGGGTGGAGGGCTGAATGAGGTCTGGGTCCTTGGCTCTTTCCAGGCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGC
PRIMER_LEFT_EXPLAIN=considered 4444, overlap target 289, low tm 844, high tm 2220, ok 1091
PRIMER_RIGHT_EXPLAIN=considered 4444, overlap target 289, low tm 400, high tm 2950, ok 805
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=2.234150
PRIMER_LEFT_0_PENALTY=0.821928
PRIMER_RIGHT_0_PENALTY=1.412222
PRIMER_LEFT_0_SEQUENCE=CCTCACTTCCATTCCTTCCC
PRIMER_RIGHT_0_SEQUENCE=CATCAGCTGACAGGAATGCTT
PRIMER_LEFT_0=278,20
PRIMER_RIGHT_0=457,21
PRIMER_LEFT_0_TM=60.822
PRIMER_RIGHT_0_TM=60.412
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=180
PRIMER_PAIR_0_PRODUCT_TM=78.9
=
SEQUENCE_ID=CETP_X4
SEQUENCE_TARGET=298,75
SEQUENCE_TEMPLATE=CTAGACAAAATTGGAGGCTCACTCCTTGGGCTCCCTGGATGACCCCCAACATCCTTCCTCACTTCCATTCCTTCCCAGCATCCAGATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGAGCTGGTGGAAGCCAAGTCCATTGATGTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGGGACCCTGAAGTATGGCTACACCACTGCCTGGTGGTAAGCATTCCTGTCAGCTGATGCCCCATGCCCTGGCCCTCTCTGGGTGGAGGGCTGAATGAGGTCTGGGTCCTTGGCTCTTTCCAGGCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGCTGGCCAAGCTCTTGACTGGCCTGGGCAGCATGTGGATACCATCTGATAGCGGAGGCTGCCCTGAGGTCATGTCGGGTCTCCCTGCAGCCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGCTACCTGTCTTTCCATAAGCTGCTCCTGCAT
PRIMER_LEFT_EXPLAIN=considered 3804, overlap target 289, low tm 382, high tm 2360, ok 773
PRIMER_RIGHT_EXPLAIN=considered 3804, overlap target 289, low tm 348, high tm 2565, ok 602
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.692433
PRIMER_LEFT_0_PENALTY=2.413192
PRIMER_RIGHT_0_PENALTY=1.279241
PRIMER_LEFT_0_SEQUENCE=GGGTCCTTGGCTCTTTCC
PRIMER_RIGHT_0_SEQUENCE=CCAGAGGACGCTTGACACAT
PRIMER_LEFT_0=280,18
PRIMER_RIGHT_0=392,20
PRIMER_LEFT_0_TM=59.587
PRIMER_RIGHT_0_TM=61.279
PRIMER_LEFT_0_GC_PERCENT=61.111
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=113
PRIMER_PAIR_0_PRODUCT_TM=76.0
=
SEQUENCE_ID=CETP_X5
SEQUENCE_TARGET=298,92
SEQUENCE_TEMPLATE=GCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGCTGGCCAAGCTCTTGACTGGCCTGGGCAGCATGTGGATACCATCTGATAGCGGAGGCTGCCCTGAGGTCATGTCGGGTCTCCCTGCAGCCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGCTACCTGTCTTTCCATAAGCTGCTCCTGCATCTCCAAGGGGAGCGAGAGTAAGTACACCACCCTGTGCCCCCATTCCTGTCGTGCCCATCCTGTTAGTGTGTCCACGGCCCCCTCCAGGCTCAACCCCACACAGGGATGCTTGTGGGTGGCCAAACCTGAGGGCAGCAATACCTTCAGTGGGGTCATTCCATCCCCCTCCATCAATACACCCTAAAGGCTGGAAACAACAATAACCAACAGCTAGTAACTAACAGCTATTAAGAACTTCTGTTGGCAAAGCACTATTCCAAGCCCTTTCATGAATTAATTGATTTTGTCCTTAAAACCAACCCTAGGATATAGATTCT
PRIMER_LEFT_EXPLAIN=considered 3974, overlap target 289, low tm 348, high tm 2707, ok 630
PRIMER_RIGHT_EXPLAIN=considered 3974, overlap target 289, GC content failed 34, low tm 889, high tm 1971, ok 791
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=6.908902
PRIMER_LEFT_0_PENALTY=4.729227
PRIMER_RIGHT_0_PENALTY=2.179675
PRIMER_LEFT_0_SEQUENCE=CATGTCGGGTCTCCCTGC
PRIMER_RIGHT_0_SEQUENCE=GGGCACAGGGTGGTGTACTT
PRIMER_LEFT_0=280,18
PRIMER_RIGHT_0=409,20
PRIMER_LEFT_0_TM=62.729
PRIMER_RIGHT_0_TM=62.180
PRIMER_LEFT_0_GC_PERCENT=66.667
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=5.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=130
PRIMER_PAIR_0_PRODUCT_TM=79.6
=
SEQUENCE_ID=CETP_X6
SEQUENCE_TARGET=298,74
SEQUENCE_TEMPLATE=TTTCTGACTCTGGAGCAAGACGGATACATGTATGAATTTGGACTCTAGACACGTTCTCGTGTGTGTGACAGGTGTGAGCGTCACAGGAGCTGGGCCCTCCCGAGGAATTCTGGATGGTGCCACAGTTAATTCTTGGGTCTGAGGCTCCGTGTTCTCACTGCAAAATGGGAGTGATAATTCTTACTTCCTGAGCTACAAGAGTCAGGGCCAACAGAGCCATGAAGGAGCCTGGTACACACTAGGCGCTCCATGGATGCACAGGACTGGTCAGGGGCTCATTGTGGTGCTTGCTGCCTTCAGGCCTGGGTGGATCAAGCAGCTGTTCACAAATTTCATCTCCTTCACCCTGAAGCTGGTCCTGAAGGGACAGGTGAGTGAGGCTGGCTGACTCCCTGTGGTCCAGGCCATGCCCAGGAGGCTGGATCCCTTTCCTCCCTGCCTTTCCCTGAGAAGGTGCCACTCCCACCTTCTCCATGTGGCCAGTCCCCTGTGCCGGTCCCCAGCACTGCCACCACCACGCAGCTGGAAGGAGGCACTCCGTCTGGCCTCCTTTCCTGCCTGGAAAGCACCTGCTCTGTCTGCCCCAGATCTGCAAAGAGATCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCTGGTGAGTGCGTTTCTGTCTGCAT
PRIMER_LEFT_EXPLAIN=considered 3794, overlap target 289, low tm 681, high tm 1966, ok 858
PRIMER_RIGHT_EXPLAIN=considered 3794, overlap target 289, low tm 274, high tm 2616, high any compl 2, high end compl 3, ok 610
PRIMER_PAIR_EXPLAIN=considered 40, high end compl 38, ok 2
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=185.393869
PRIMER_LEFT_0_PENALTY=82.014189
PRIMER_RIGHT_0_PENALTY=103.379680
PRIMER_LEFT_0_SEQUENCE=CATTGTGGTGCTTGCTGC
PRIMER_RIGHT_0_SEQUENCE=ACAGGGAGTCAGCCAGCCT
PRIMER_LEFT_0=276,18
PRIMER_RIGHT_0=395,19
PRIMER_LEFT_0_TM=59.986
PRIMER_RIGHT_0_TM=62.380
PRIMER_LEFT_0_GC_PERCENT=55.556
PRIMER_RIGHT_0_GC_PERCENT=63.158
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=80.000000
PRIMER_RIGHT_0_POSITION_PENALTY=100.000000
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=120
PRIMER_PAIR_0_PRODUCT_TM=77.5
=
SEQUENCE_ID=CETP_X7
SEQUENCE_TARGET=298,65
SEQUENCE_TEMPLATE=TTGCTGCCTTCAGGCCTGGGTGGATCAAGCAGCTGTTCACAAATTTCATCTCCTTCACCCTGAAGCTGGTCCTGAAGGGACAGGTGAGTGAGGCTGGCTGACTCCCTGTGGTCCAGGCCATGCCCAGGAGGCTGGATCCCTTTCCTCCCTGCCTTTCCCTGAGAAGGTGCCACTCCCACCTTCTCCATGTGGCCAGTCCCCTGTGCCGGTCCCCAGCACTGCCACCACCACGCAGCTGGAAGGAGGCACTCCGTCTGGCCTCCTTTCCTGCCTGGAAAGCACCTGCTCTGTCTGCCCCAGATCTGCAAAGAGATCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCTGGTGAGTGCGTTTCTGTCTGCATGCCTCAGAAGACAGCAGTGGGAGCCAGAAAGCCACCTGCTGCACTATGTGGCCTTGGGACTGTCACTCTTCCTGTCTAGGTCCCATGGGCTCTATCTGGCTCTGACACTTGATGATTAGTTATGAGCATACTTTGGCAAAGCTCTGCCCCTTTGGTGCGGCTCACAAGCTGTGTGGCGAAGGGCTTGTCTATAGAACTCAGGACAAATGGGTGATTAAGTCCAAGAGGCATCCAAGATTCTCCTGGAAGTAGATTAGGAAAAAAGATAATTAGATTGC
PRIMER_LEFT_EXPLAIN=considered 3704, overlap target 289, low tm 262, high tm 2572, high end compl 3, ok 578
PRIMER_RIGHT_EXPLAIN=considered 3593, overlap target 289, GC content failed 15, low tm 752, high tm 1654, long poly-x seq 22, ok 861
PRIMER_PAIR_EXPLAIN=considered 102, high any compl 81, high end compl 20, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=141.548415
PRIMER_LEFT_0_PENALTY=140.588967
PRIMER_RIGHT_0_PENALTY=0.959447
PRIMER_LEFT_0_SEQUENCE=CTGGAAAGCACCTGCTCTGT
PRIMER_RIGHT_0_SEQUENCE=ATGCAGACAGAAACGCACTC
PRIMER_LEFT_0=271,20
PRIMER_RIGHT_0=382,20
PRIMER_LEFT_0_TM=60.589
PRIMER_RIGHT_0_TM=59.041
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=140.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=112
PRIMER_PAIR_0_PRODUCT_TM=75.4
=
SEQUENCE_ID=CETP_X8
SEQUENCE_TARGET=298,96
SEQUENCE_TEMPLATE=GCTGGGCACTCATCCATGTACTGTACTCTCCTATGCAGTACAGAGCAGAGCTGGGTTTCAGCCCAAGTCTTGGACTCTGCTCTGAACCAACCTTCTAGAAGGGCTCTACCTACCCAGACAGACAGACTTGGGAAAAGAGAGAATGAAAAAGTGCCACACCCCTCCCCGCACACCCAGGTCCCACTTTACAGAGGGGAACACTGAGGCTGGAGGGTTGGGTAGCTGTGTGGATGCAGGGGACGGTGACTCAGGGCAATTCCCCCATCCCTGAGGCCCTGCGTTGATCTTTTCCTCCTGCAGCCAGCATCCTTTCAGATGGAGACATTGGGGTGGACATTTCCCTGACAGGTGATCCCGTCATCACAGCCTCCTACCTGGAGTCCCATCACAAGGTAGGAGTTGTGGGAGGGTGGGCAGGGCCCAGCTTCCCCAGGGGAGTTGGTCCTTTTTTGTGCTCTGACAACCCCATCCCCCAGCTTCAACCTTATGGCAGCCAAGAGTCCTGGGGAGCTCCTCCTCATTCCTGATGCTCCTCCGCATTCCTGATGCTGCGAGGAGGGCAGGCCACAGCGACGTGCCCCTGACCCCTCTCTGCAGGCACCAGGGCTGCCCACTACAAGGATCCCAGCAAAGCACCAGCTCCTTCCTAGAGGGCTTATTCGGCTTCTGTCATCCTCTACAGCAGTGGATTG
PRIMER_LEFT_EXPLAIN=considered 4014, overlap target 289, low tm 645, high tm 2223, ok 857
PRIMER_RIGHT_EXPLAIN=considered 3861, overlap target 289, GC content failed 8, low tm 269, high tm 2629, long poly-x seq 22, ok 644
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=3.514067
PRIMER_LEFT_0_PENALTY=0.186600
PRIMER_RIGHT_0_PENALTY=3.327467
PRIMER_LEFT_0_SEQUENCE=CGTTGATCTTTTCCTCCTGC
PRIMER_RIGHT_0_SEQUENCE=CCACCCTCCCACAACTCCT
PRIMER_LEFT_0=278,20
PRIMER_RIGHT_0=412,19
PRIMER_LEFT_0_TM=59.813
PRIMER_RIGHT_0_TM=62.327
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=63.158
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=1.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=135
PRIMER_PAIR_0_PRODUCT_TM=77.9
=
SEQUENCE_ID=CETP_X9
SEQUENCE_TARGET=163,184
SEQUENCE_TEMPLATE=TTTGTTGAATGAGTGAAAGCCGCTGGGGAAACTGGGTACAGCTCTTTCCTCAGTTTCCCCATCTGCACTCTGGGCTGAATGCTGGGCTCCTCCCAATCTCCCTGAAGCTGGACCTGAGCCCAGTAGGGACACACAGGGTCCAGCCAGCGTCCTGGCTTCCTCCAGGGTCATTTCATCTACAAGAATGTCTCAGAGGACCTCCCCCTCCCCACCTTCTCGCCCACACTGCTGGGGGACTCCCGCATGCTGTACTTCTGGTTCTCTGAGCGAGTCTTCCACTCGCTGGCCAAGGTAGCTTTCCAGGATGGCCGCCTCATGCTCAGCCTGATGGGAGACGAGTTCAAGGTGAGTGGGTGGGGCTGGGCTGCTAGGGGATCCAGATGGCATGTGGTATGTGTGTGTGTGCACACGCATGGGGAGGAGGGAGGAAACTCGGAAACTTGGTGGTGGGCAAAAGAACTAAGCTGGAGCAATAGCAGTGAAGTCCAGACTGGGCACAGTGGCTCACACCTGTAATCCCAATCCTTTGGGAGGCTGAGATGTAGCAGGACGAACCGCAGACAAAACTCCTCAGACACTGAGTTAAAGAAGGAAAGAGTTTATTCAGCCGGGAGCATGGGTAAGACTC
PRIMER_LEFT_EXPLAIN=considered 3527, overlap target 272, low tm 323, high tm 2355, ok 577
PRIMER_RIGHT_EXPLAIN=considered 4589, overlap target 154, low tm 711, high tm 2838, ok 886
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=229.497858
PRIMER_LEFT_0_PENALTY=4.864632
PRIMER_RIGHT_0_PENALTY=224.633226
PRIMER_LEFT_0_SEQUENCE=AGCGTCCTGGCTTCCTCC
PRIMER_RIGHT_0_SEQUENCE=ATCCCCTAGCAGCCCAGC
PRIMER_LEFT_0=145,18
PRIMER_RIGHT_0=375,18
PRIMER_LEFT_0_TM=62.865
PRIMER_RIGHT_0_TM=62.633
PRIMER_LEFT_0_GC_PERCENT=66.667
PRIMER_RIGHT_0_GC_PERCENT=66.667
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, reverse MIR2 Repetitive element - a consensus
PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=220.000000
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=231
PRIMER_PAIR_0_PRODUCT_TM=81.8
=
SEQUENCE_ID=CETP_X10
SEQUENCE_TARGET=235,55
SEQUENCE_TEMPLATE=CTTGTCGAGGTCACACAGCATGTGTGGGGCACAGTGGGGAGCAGAAGCCAGCCTCCAGCCGGACACCCCTTCCCCATTCCACACCCTGCCCAGAGCATCTCACATGTTGTCTGGGAGGTTGGGAGTTGCGTCTGAGGAGGGGTCCAGTCCTTGAAACTGCCCTTGGTCCCTGCGAAGTTTTCTTCTGAGGAGTGGACTTTACTCCACCCACCCTCCAACTTCCTCATTTCTTTTCAGGCAGTGCTGGAGACCTGGGGCTTCAACACCAACCAGGAAATCTTCCAAGAGGTAACTCCCCCCTGCCCCTGTGTGGGGTTTATCTCACGTACCCCAATCCTGCTCTGGCTTCAAGAGCCCCCACACAGCCAATAACACCACCAATGGCAACAATTATAACAG
PRIMER_LEFT_EXPLAIN=considered 2785, overlap target 100, low tm 259, high tm 1906, ok 520
PRIMER_RIGHT_EXPLAIN=considered 1498, overlap target 226, low tm 153, high tm 792, long poly-x seq 22, ok 305
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=227.452455
PRIMER_LEFT_0_PENALTY=4.533349
PRIMER_RIGHT_0_PENALTY=222.919106
PRIMER_LEFT_0_SEQUENCE=TCCAACTTCCTCATTTCTTTTC
PRIMER_RIGHT_0_SEQUENCE=ATAAACCCCACACAGGGGC
PRIMER_LEFT_0=213,22
PRIMER_RIGHT_0=319,19
PRIMER_LEFT_0_TM=57.467
PRIMER_RIGHT_0_TM=61.919
PRIMER_LEFT_0_GC_PERCENT=36.364
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=220.000000
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=12.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=107
PRIMER_PAIR_0_PRODUCT_TM=75.8
=
SEQUENCE_ID=CETP_X11
SEQUENCE_TARGET=125,169
SEQUENCE_TEMPLATE=CCCCGAGCTACTTCCTTTTCCCAGCCCTGGGGCCCGGAGCCAGCTTTGTGGTTCCCATCTCCGAGGGCATGGACTGCTGCTCCGTGATGGGCCCCTGTCCTGGCCATGGGACCCCTGTCTTCCACAGGTTGTCGGCGGCTTCCCCAGCCAGGCCCAAGTCACCGTCCACTGCCTCAAGATGCCCAAGATCTCCTGCCAAAACAAGGGAGTCGTGGTCAATTCTTCAGTGATGGTGAAATTCCTCTTTCCACGCCCAGACCAGCAACATTCTGTAGCTTACACATTTGAAGAGGTGAGGCGGGTGCAGGGAGAGGTGGTGGTGGGGGAACTGACTCACATATGGGCCGCAGAGGGCAGGGGCTGGGGGTCTCTGAAGCCTCCAGATCCTTCTCACCACCTCTGCTGGCAC
PRIMER_LEFT_EXPLAIN=considered 2831, overlap target 106, GC content failed 50, low tm 286, high tm 1982, ok 407
PRIMER_RIGHT_EXPLAIN=considered 2741, overlap target 116, GC content failed 42, low tm 336, high tm 1775, ok 472
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=125.433613
PRIMER_LEFT_0_PENALTY=2.311131
PRIMER_RIGHT_0_PENALTY=123.122481
PRIMER_LEFT_0_SEQUENCE=GGGACCCCTGTCTTCCAC
PRIMER_RIGHT_0_SEQUENCE=ACCACCTCTCCCTGCACC
PRIMER_LEFT_0=107,18
PRIMER_RIGHT_0=317,18
PRIMER_LEFT_0_TM=60.311
PRIMER_RIGHT_0_TM=61.122
PRIMER_LEFT_0_GC_PERCENT=66.667
PRIMER_RIGHT_0_GC_PERCENT=66.667
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=120.000000
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=211
PRIMER_PAIR_0_PRODUCT_TM=80.0
=
SEQUENCE_ID=CETP_X12
SEQUENCE_TARGET=298,72
SEQUENCE_TEMPLATE=GTTGCAGTGAGCTGAGATCGTGCCACTGCCCTCCAGCCTGGGCAACAGAGTGAGACTGTCTCAAAAACAAAAAAAGAAAAGAAAAGAAAAAGAAAGTGACTTCTCAGGTCCTAACCCCAAAGCCACAGGTGCTGGGGAACTTTCCTCGGTTTTCAGAAGAGCAGTAGCTAAGCCTGGTTCCCGTGTCATCCTTGCCTCTCCAGTCCCTCAGTGGAAAGAATCAGGGGCCCTGAGCTAGGAGGGTTGCTCTCTGCTTCGGGAAGAGCCCTGGCTCACAGCAAATTTGGTTTCTCTCCCCAGGATATCGTGACTACCGTCCAGGCCTCCTATTCTAAGAAAAAGCTCTTCTTAAGCCTCTTGGATTTCCAGTATGTGCTGCAGAGAAGAGAAGGGGGCGGTCAACTCCGCAAACCTCTCCCTGGCCCCTTGGAGTCAGGCACAGGGCGGGGTGTTGGTGGGGAAATGTGGCCCCTTTCTTCTGGGGCATATGGGCTGACTGCAGGGAAGATAAGACCCTGCCTAGATAGAATCTTCGTGGGGAAGAAGGGGCTCCAGGAAGAATGGAGGGCTGCCAGGAAGAAGGCCTGAGCTATGAGACAAAAGCACTGGCTGCTATTCTTAGAGTTTCTTTCCCAGGGGATGTTACAGGAGGGGGCCCAATGGAGGGT
PRIMER_LEFT_EXPLAIN=considered 3686, overlap target 289, GC content failed 114, low tm 669, high tm 1809, long poly-x seq 14, ok 791
PRIMER_RIGHT_EXPLAIN=considered 3774, overlap target 289, low tm 619, high tm 2094, ok 772
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=4.608119
PRIMER_LEFT_0_PENALTY=1.522783
PRIMER_RIGHT_0_PENALTY=3.085335
PRIMER_LEFT_0_SEQUENCE=CAAATTTGGTTTCTCTCCCC
PRIMER_RIGHT_0_SEQUENCE=CCTTCTCTTCTCTGCAGCACAT
PRIMER_LEFT_0=278,20
PRIMER_RIGHT_0=391,22
PRIMER_LEFT_0_TM=58.477
PRIMER_RIGHT_0_TM=61.085
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=10.9000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=114
PRIMER_PAIR_0_PRODUCT_TM=73.3
=
SEQUENCE_ID=CETP_X13
SEQUENCE_TARGET=298,38
SEQUENCE_TEMPLATE=CGGTCAACTCCGCAAACCTCTCCCTGGCCCCTTGGAGTCAGGCACAGGGCGGGGTGTTGGTGGGGAAATGTGGCCCCTTTCTTCTGGGGCATATGGGCTGACTGCAGGGAAGATAAGACCCTGCCTAGATAGAATCTTCGTGGGGAAGAAGGGGCTCCAGGAAGAATGGAGGGCTGCCAGGAAGAAGGCCTGAGCTATGAGACAAAAGCACTGGCTGCTATTCTTAGAGTTTCTTTCCCAGGGGATGTTACAGGAGGGGGCCCAATGGAGGGTCAAATTATCATCGCTTTTTTATTTCAGGATTACACCAAAGACTGTTTCCAACTTGACTGAGGTAGGTAGTCTTGGATAGACTGGGGGAAATAAGTCCTGTGGGACCTCCTGCCTTAAAGAAAGCAGGCGGAGGGCCCTAAAGGAAATCAGGCAACCAGACCAAAAGAATGTGACCAGGTGGTCCATGCTGTGTCTCTTGTGACCCTTCTTCTCCCTGCCATGTCTTTTGGGAGAGCCCTTGTGTTGCAAAAATGAGAGTGTGGTGGTATGGATTGGGGTTTAGGCAGAACAGTACTGGCCAAGCAGCGCTCCCTGGACCTCAATTTTCCCTCTGTGGAATGGGCTAGCAATCCTGGGCCTC
PRIMER_LEFT_EXPLAIN=considered 3282, overlap target 289, GC content failed 1, low tm 523, high tm 1815, long poly-x seq 22, ok 632
PRIMER_RIGHT_EXPLAIN=considered 3434, overlap target 289, low tm 586, high tm 1682, ok 877
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=126.624750
PRIMER_LEFT_0_PENALTY=123.526785
PRIMER_RIGHT_0_PENALTY=3.097964
PRIMER_LEFT_0_SEQUENCE=GGGTCAAATTATCATCGCTTTTT
PRIMER_RIGHT_0_SEQUENCE=CCCCAGTCTATCCAAGACTACCT
PRIMER_LEFT_0=269,23
PRIMER_RIGHT_0=358,23
PRIMER_LEFT_0_TM=60.527
PRIMER_RIGHT_0_TM=59.902
PRIMER_LEFT_0_GC_PERCENT=34.783
PRIMER_RIGHT_0_GC_PERCENT=52.174
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=120.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=90
PRIMER_PAIR_0_PRODUCT_TM=70.1
=
SEQUENCE_ID=CETP_X14
SEQUENCE_TARGET=298,77
SEQUENCE_TEMPLATE=GTTGCAAAAATGAGAGTGTGGTGGTATGGATTGGGGTTTAGGCAGAACAGTACTGGCCAAGCAGCGCTCCCTGGACCTCAATTTTCCCTCTGTGGAATGGGCTAGCAATCCTGGGCCTCCCCAGGGCGAAGGAAAGACCACTCAGGAAGGGCACCGTCTGGGGCAGGAAAACGGAGTGGGTTGGATGTATTTTTTTCACGGATGGGCATGAGGATGAATGCTTGTCCAGGCCGTGCAGCATCTGCCTTGTGGGTCACTTCTGTGCTCCAGGGAGGACTCACCATGGGCATTTGATTGCAGAGCAGCTCCGAGTCCGTCCAGAGCTTCCTGCAGTCAATGATCACCGCTGTGGGCATCCCTGAGGTCATGTCTCGTAAGTGTGGGCTGGAGGGGAAACTGGGTGCCGAGGCTGACAGAGCTTCCCATTTCACCTTTT
PRIMER_LEFT_EXPLAIN=considered 3425, overlap target 52, GC content failed 3, low tm 235, high tm 2489, high end compl 2, long poly-x seq 23, ok 621
PRIMER_RIGHT_EXPLAIN=considered 1454, overlap target 289, low tm 83, high tm 882, ok 200
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=106.573018
PRIMER_LEFT_0_PENALTY=21.740693
PRIMER_RIGHT_0_PENALTY=84.832325
PRIMER_LEFT_0_SEQUENCE=CACCATGGGCATTTGATTG
PRIMER_RIGHT_0_SEQUENCE=GTTTCCCCTCCAGCCCAC
PRIMER_LEFT_0=278,19
PRIMER_RIGHT_0=396,18
PRIMER_LEFT_0_TM=60.741
PRIMER_RIGHT_0_TM=62.832
PRIMER_LEFT_0_GC_PERCENT=47.368
PRIMER_RIGHT_0_GC_PERCENT=66.667
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=20.000000
PRIMER_RIGHT_0_POSITION_PENALTY=80.000000
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=119
PRIMER_PAIR_0_PRODUCT_TM=78.3
=
SEQUENCE_ID=CETP_X15
SEQUENCE_TARGET=254,90
SEQUENCE_TEMPLATE=GGATGGGTTGGGAGCTCAAGTTTTGGGGCAGAAGGGAATTTTTTTTGGCAGCAGAGTGCAAGCCCTGCCGCCAGGCAAACTCTGCTCTTCCTCATCCTCAGAAGCACTTGCTCACTCTGCTAAATCAAAGTGAAACGCATGTTTACAGAATATTGGTCCAAAAGGGTCTCAGCATCTCCCACTACCCAGGGTGCAGAGCCTCGGGCCGGCCTTGCTCCCCAAGAAGGGCTGACTGGGGCTCTGTCCCCTCGCCCAGGGCTCGAGGTAGTGTTTACAGCCCTCATGAACAGCAAAGGCGTGAGCCTCTTCGACATCATCAACCCTGAGATTATCACTCGAGATGTGAGTACAAAGCCCCCCTCACCAGCCCCTGTTCCTGGGGAGAGAGGCCCAGACAGGATTCCTGGGGTGACTGGGGGCTGTTGGGGAGACAGACAGAGGGGCCTCTACCAGCTTGGCTCCCTCCTGGTGGCCTGGGAGTCAGCCCAGCTCGCCCCTCTCTCCTACTGCCCCTCCCTTCAGGGCTTCCTGCTGCTGCAGATGGACTTTGGCTTCCCTGAGCACCTGCTGGTGGATTTCCTCCAGAGCTTGAGCTAGAAGTCTCCAAGGAGGTCGGGATGGGGCTTGTAGCAGAAGGCAAGC
PRIMER_LEFT_EXPLAIN=considered 3343, overlap target 289, GC content failed 24, low tm 488, high tm 1840, long poly-x seq 24, ok 678
PRIMER_RIGHT_EXPLAIN=considered 3758, overlap target 245, GC content failed 1, low tm 356, high tm 2464, high repeat similarity 3, long poly-x seq 22, ok 667
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=427.731805
PRIMER_LEFT_0_PENALTY=104.061036
PRIMER_RIGHT_0_PENALTY=323.670770
PRIMER_LEFT_0_SEQUENCE=ACTGGGGCTCTGTCCCCT
PRIMER_RIGHT_0_SEQUENCE=AGGAACAGGGGCTGGTGA
PRIMER_LEFT_0=231,18
PRIMER_RIGHT_0=377,18
PRIMER_LEFT_0_TM=62.061
PRIMER_RIGHT_0_TM=61.671
PRIMER_LEFT_0_GC_PERCENT=66.667
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=100.000000
PRIMER_RIGHT_0_POSITION_PENALTY=320.000000
PRIMER_LEFT_0_END_STABILITY=10.9000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=147
PRIMER_PAIR_0_PRODUCT_TM=79.0
=
SEQUENCE_ID=CETP_X16
SEQUENCE_TARGET=298,79
SEQUENCE_TEMPLATE=GAAGGGCTGACTGGGGCTCTGTCCCCTCGCCCAGGGCTCGAGGTAGTGTTTACAGCCCTCATGAACAGCAAAGGCGTGAGCCTCTTCGACATCATCAACCCTGAGATTATCACTCGAGATGTGAGTACAAAGCCCCCCTCACCAGCCCCTGTTCCTGGGGAGAGAGGCCCAGACAGGATTCCTGGGGTGACTGGGGGCTGTTGGGGAGACAGACAGAGGGGCCTCTACCAGCTTGGCTCCCTCCTGGTGGCCTGGGAGTCAGCCCAGCTCGCCCCTCTCTCCTACTGCCCCTCCCTTCAGGGCTTCCTGCTGCTGCAGATGGACTTTGGCTTCCCTGAGCACCTGCTGGTGGATTTCCTCCAGAGCTTGAGCTAGAAGTCTCCAAGGAGGTCGGGATGGGGCTTGTAGCAGAAGGCAAGCACCAGGCTCACAGCTGGAACCCTGGTGTCTCCTCCAGCGTGGTGGAAGTTGGGTTAGGAGTACGGAGATGGAGATTGGCTCCCAACTCCTCCCTATCCTAAAGGCCCACTGGCATTAAAGTGCTGTATCCAAGAGCTGCGGAGTCCTTCTTCTGTGGCTGGCGGGTAGAGGGGGGGGGAAGGGATTGTCTCACCAGTGCCGTCCACCTCTTTTCAGCCCTTCCAAGCAGCTGCCCCCAAACCCTCCAAGCTT
PRIMER_LEFT_EXPLAIN=considered 3689, overlap target 286, GC content failed 3, low tm 288, high tm 2492, long poly-x seq 22, ok 598
PRIMER_RIGHT_EXPLAIN=considered 3637, overlap target 289, GC content failed 3, low tm 299, high tm 2316, long poly-x seq 25, ok 705
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=4.990886
PRIMER_LEFT_0_PENALTY=2.381034
PRIMER_RIGHT_0_PENALTY=2.609852
PRIMER_LEFT_0_SEQUENCE=CTCCTACTGCCCCTCCCTTC
PRIMER_RIGHT_0_SEQUENCE=CCCGACCTCCTTGGAGAC
PRIMER_LEFT_0=278,20
PRIMER_RIGHT_0=394,18
PRIMER_LEFT_0_TM=62.381
PRIMER_RIGHT_0_TM=60.610
PRIMER_LEFT_0_GC_PERCENT=65.000
PRIMER_RIGHT_0_GC_PERCENT=66.667
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=117
PRIMER_PAIR_0_PRODUCT_TM=79.0
=
SEQUENCE_ID=F2_X1
SEQUENCE_TARGET=298,114
SEQUENCE_TEMPLATE=AAGCAACCACCGCTATATGTTAGGATTCGAAGGAGCTCCAGGAAAGTCTCATAGCCCCACTGGCCAGAATGGGCTAAATCTCAGAGGGGGAGGGTGGGAGATGGGGGTGACAGTGACCTTTTTTGTGACTCCTCCTAGACCATCCATCCCTGCTCCCAGGAGGACCTGTCCTCCCAGATGGTGGAGATGGACAGGAGGACTATCTACCCACCCGTCCCCACGGCCCTGACCCTCTGACCTCACCCTCTCCGCTGATTTCTTCATGTTAGTTCAACATTACCCAGAGGGGTCAGGACAGACAATTCCTCAGTGACCCAGGAGCTGACACACTATGGCGCACGTCCGAGGCTTGCAGCTGCCTGGCTGCCTGGCCCTGGCTGCCCTGTGTAGCCTTGTGCACAGCCAGCATGGTAAGGGAGTGCTTGCAGGCTGGAACAGGCTGGAGGACTGGGGTGTGGGCCCATGGGCTGGGGTCTCCTGGCTGGACAGAGCACACAGAGCTGGCCCCTAAGTAGGTCTCAGCCCCAGGCGGCCAGCTTAGGGAAGAAGTCAGGAGCTCAGGGCTGGAAAGAGAATGGCTGCTTCTCTCTTCCAATATAGGGAGCAGGCTGGGGGCAAGGGGCAGTGTAGGAGGGGCACAGGGGGCCACATTTAGCAGCCTTCCAGGCCTTCCACCAGCCCAGACTGCCTCTCTCAGAAGCCAGCAGGGG
PRIMER_LEFT_EXPLAIN=considered 4041, overlap target 289, GC content failed 21, low tm 498, high tm 2476, long poly-x seq 22, ok 735
PRIMER_RIGHT_EXPLAIN=considered 4194, overlap target 289, GC content failed 8, low tm 235, high tm 3082, high repeat similarity 2, ok 578
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=25.987217
PRIMER_LEFT_0_PENALTY=2.861995
PRIMER_RIGHT_0_PENALTY=23.125223
PRIMER_LEFT_0_SEQUENCE=CCAGAGGGGTCAGGACAG
PRIMER_RIGHT_0_SEQUENCE=AGCCTGCAAGCACTCCCT
PRIMER_LEFT_0=280,18
PRIMER_RIGHT_0=430,18
PRIMER_LEFT_0_TM=59.138
PRIMER_RIGHT_0_TM=61.125
PRIMER_LEFT_0_GC_PERCENT=66.667
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=20.000000
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=151
PRIMER_PAIR_0_PRODUCT_TM=81.5
=
SEQUENCE_ID=F2_X2
SEQUENCE_TARGET=298,165
SEQUENCE_TEMPLATE=AGAGCTGGCCCCTAAGTAGGTCTCAGCCCCAGGCGGCCAGCTTAGGGAAGAAGTCAGGAGCTCAGGGCTGGAAAGAGAATGGCTGCTTCTCTCTTCCAATATAGGGAGCAGGCTGGGGGCAAGGGGCAGTGTAGGAGGGGCACAGGGGGCCACATTTAGCAGCCTTCCAGGCCTTCCACCAGCCCAGACTGCCTCTCTCAGAAGCCAGCAGGGGAGGGTGGGCTTGCTTCATGCCCCCAGATGGCCAAGACTGCCTGTTCCTGAGGTCGCTGTTCCATGACCCCCCCACCGCCTTTACAGTGTTCCTGGCTCCTCAGCAAGCACGGTCGCTGCTCCAGCGGGTCCGGCGAGCCAACACCTTCTTGGAGGAGGTGCGCAAGGGCAACCTAGAGCGAGAGTGCGTGGAGGAGACGTGCAGCTACGAGGAGGCCTTCGAGGCTCTGGAGTCCTCCACGGCTACGGTGAGCCTGGGCTGCTCGGACGGTGCCGGGGCCTCAGACCGGGCCCAACTCTAGACACTTCCACAGAGAAGCAAGCGAGGAACGCCACAGCCCCTTCGCTGCTCACAGCCTCATTTCAACTCTGAGCCCCTCCTCACAGGGCTGGCAAGAGGAGCGGCCTCAGCCTTTCCTGGGGGTCTCTGTGCCTGGACTGTGTCCCTGTGCAGCTCCATGACATGGGGAGGCCTCCACAGTCTTCAGACATCCACCTGCCTTGGAGCTCTGTGTCCACATGGCCTCCTCAGCGGCAGACTCCCACAC
PRIMER_LEFT_EXPLAIN=considered 4542, overlap target 289, GC content failed 14, low tm 245, high tm 3332, long poly-x seq 23, ok 639
PRIMER_RIGHT_EXPLAIN=considered 4704, overlap target 289, GC content failed 96, low tm 191, high tm 3583, high end compl 4, ok 541
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=1004.486617
PRIMER_LEFT_0_PENALTY=302.054062
PRIMER_RIGHT_0_PENALTY=702.432556
PRIMER_LEFT_0_SEQUENCE=GTCGCTGTTCCATGACCC
PRIMER_RIGHT_0_SEQUENCE=GTCTAGAGTTGGGCCCGGT
PRIMER_LEFT_0=265,18
PRIMER_RIGHT_0=516,19
PRIMER_LEFT_0_TM=60.054
PRIMER_RIGHT_0_TM=61.433
PRIMER_LEFT_0_GC_PERCENT=61.111
PRIMER_RIGHT_0_GC_PERCENT=63.158
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=7.00, reverse ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_PAIR_0_LIBRARY_MISPRIMING=15.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=300.000000
PRIMER_RIGHT_0_POSITION_PENALTY=700.000000
PRIMER_LEFT_0_END_STABILITY=9.1000
PRIMER_RIGHT_0_END_STABILITY=11.1000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=252
PRIMER_PAIR_0_PRODUCT_TM=84.5
=
SEQUENCE_ID=F2_X3
SEQUENCE_TARGET=298,29
SEQUENCE_TEMPLATE=TGTTCCTGGAGCTCTGTGTCGCCTTTCCTGTCTGTAGGGACTCTGCCAGGGACCCACTGCCCCCTCTCCTCCCATCTCCCCCAGCCTCTTTCAGACTCGGTGTGTGTGTTGGAGGAACTCCCCTATCCTCAAATATTCTTCTCCTTTTGGAAACAAAAGTAGGAAACTCTGCCACAAACCTCCCCAGAGCCTGCCCCCTGCGTGACCAGGGTAAGGAAAGTGTGAGGAGGAGCATAACATTTACTAAAACAACACAAAACAGGAGCTGCCGTAGCCTCACTCCCAGCCCTTGTTTTTCAGGATGTGTTCTGGGCCAAGTACACAGGTGAGCACCGGGAAGGATTTGCCCCAGGAAGGGAGGCCTGGGGACCCCAGTGAGAGAATTCTACCCAGAGAATCTTCTGCTGCACCTAGCCATCCACCCATCCACCCCTTCCCCACTCCTTCCTTGGTCCCTCCCATCTGTTCATCCATCTTTCTGTTTCTCACCAACATCCCATCCACCCTGACTCCAGCTCATCCTGGCCATACCCCAATCCCAAAGGTAAACACCTGGGTCTTTTCCAGCTTGTGAGACAGCGAGGACGCCTCGAGATAAGCTTGCTGCATGTCTGGAAGGTGAGCA
PRIMER_LEFT_EXPLAIN=considered 3344, overlap target 289, GC content failed 7, low tm 659, high tm 1679, ok 710
PRIMER_RIGHT_EXPLAIN=considered 3344, overlap target 289, GC content failed 1, low tm 379, high tm 2040, ok 635
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=2.486407
PRIMER_LEFT_0_PENALTY=0.475706
PRIMER_RIGHT_0_PENALTY=2.010701
PRIMER_LEFT_0_SEQUENCE=ACTCCCAGCCCTTGTTTTTC
PRIMER_RIGHT_0_SEQUENCE=AAATCCTTCCCGGTGCTC
PRIMER_LEFT_0=278,20
PRIMER_RIGHT_0=344,18
PRIMER_LEFT_0_TM=60.476
PRIMER_RIGHT_0_TM=60.011
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.556
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=67
PRIMER_PAIR_0_PRODUCT_TM=72.4
=
SEQUENCE_ID=F2_X4
SEQUENCE_TARGET=298,55
SEQUENCE_TEMPLATE=GCCGTAGCCTCACTCCCAGCCCTTGTTTTTCAGGATGTGTTCTGGGCCAAGTACACAGGTGAGCACCGGGAAGGATTTGCCCCAGGAAGGGAGGCCTGGGGACCCCAGTGAGAGAATTCTACCCAGAGAATCTTCTGCTGCACCTAGCCATCCACCCATCCACCCCTTCCCCACTCCTTCCTTGGTCCCTCCCATCTGTTCATCCATCTTTCTGTTTCTCACCAACATCCCATCCACCCTGACTCCAGCTCATCCTGGCCATACCCCAATCCCAAAGGTAAACACCTGGGTCTTTTCCAGCTTGTGAGACAGCGAGGACGCCTCGAGATAAGCTTGCTGCATGTCTGGAAGGTGAGCAACTGACACGGGTTTGGGGAGCAGGACATGGAGGGGAGCTTGGGAGAAGAGCTCAGGGGTGGGTTTGGAGTGTGGCTGGTGGAGGCCGAGGCAGTCCCCAGCATCTGACATTGCTCCCATTCCTGGGGTCAAGATGTCTCTTTGTACCTGGCTCTGTGTCTGGCATGCGAACGAATGAATGAATGAATGGACTAATGAATTAATGTTTTTTTTTTTGAGACAGAGTCTCGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCACGATCTTGGCTCACTGTAAACTCCGCCTCCCGGA
PRIMER_LEFT_EXPLAIN=considered 3604, overlap target 289, GC content failed 1, low tm 409, high tm 2209, high any compl 3, high end compl 14, ok 679
PRIMER_RIGHT_EXPLAIN=considered 3497, overlap target 289, GC content failed 107, low tm 371, high tm 2132, long poly-x seq 18, ok 580
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=123.319487
PRIMER_LEFT_0_PENALTY=121.626330
PRIMER_RIGHT_0_PENALTY=1.693157
PRIMER_LEFT_0_SEQUENCE=CCAAAGGTAAACACCTGGGTC
PRIMER_RIGHT_0_SEQUENCE=CAAACCCGTGTCAGTTGCTC
PRIMER_LEFT_0=271,21
PRIMER_RIGHT_0=372,20
PRIMER_LEFT_0_TM=60.626
PRIMER_RIGHT_0_TM=61.693
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=15.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=120.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=9.1000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=102
PRIMER_PAIR_0_PRODUCT_TM=75.7
=
SEQUENCE_ID=F2_X5
SEQUENCE_TARGET=298,110
SEQUENCE_TEMPLATE=TGCTGGCCAAGAGAAGCCACAGAATGGAAGCTCCATGAGGGCAGGGCTGTGACTGTCCTATTGGTTGATGTGTACTGAGCACCCGACAGTGCCTGTCATATGGTAGGCACTTAGCGAATATTTGGAGGCCACTGTTGAGTGAATGGGAGAACTGCTGGTTGCAGAGGAAGAGGGGCTGGGTGAATGCAGGTTCAGGATTGTGGACCTGCATGAGCTGGGAGGTGGGGGATAGACAACTTTGCAGGGAGAGAGGAAATAAGTCCCCAGGCTCCAAGGCTGACCGGGGTGGGGTCTCCGCAGGTAACTGTGCTGAGGGTCTGGGTACGAACTACCGAGGGCATGTGAACATCACCCGGTCAGGCATTGAGTGCCAGCTATGGAGGAGTCGCTACCCACATAAGCCTGAGTGAGTGAGGGGTCGGCCTTCCCACCATGGGCTGAGAACAGGGAGCAAGCGTACCTCAAGTTCAACAGCCTCCTGTTGGGCAATTTCCTCTTCCAGAATCAACTCCACTACCCATCCTGGGGCCGACCTACAGGAGAATTTCTGCCGCAACCCCGACAGCAGCACCACGGGACCCTGGTGCTACACTACAGACCCCACCGTGAGGAGGCAGGAATGCAGCATCCCTGTCTGTGGTAGGCTGGGGGCAGTGGGGCGACCCATGACCAAGCCCGGGGGCTTCATGGGGCCTGGCAGCCTGGG
PRIMER_LEFT_EXPLAIN=considered 4154, overlap target 289, GC content failed 2, low tm 468, high tm 2494, ok 901
PRIMER_RIGHT_EXPLAIN=considered 4154, overlap target 289, GC content failed 14, low tm 310, high tm 2916, ok 625
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=328.554484
PRIMER_LEFT_0_PENALTY=323.965248
PRIMER_RIGHT_0_PENALTY=4.589237
PRIMER_LEFT_0_SEQUENCE=CAGGCTCCAAGGCTGACC
PRIMER_RIGHT_0_SEQUENCE=AAGGCCGACCCCTCACTC
PRIMER_LEFT_0=264,18
PRIMER_RIGHT_0=425,18
PRIMER_LEFT_0_TM=61.965
PRIMER_RIGHT_0_TM=62.589
PRIMER_LEFT_0_GC_PERCENT=66.667
PRIMER_RIGHT_0_GC_PERCENT=66.667
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=320.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=162
PRIMER_PAIR_0_PRODUCT_TM=81.0
=
SEQUENCE_ID=F2_X6
SEQUENCE_TARGET=298,141
SEQUENCE_TEMPLATE=GACCTGCATGAGCTGGGAGGTGGGGGATAGACAACTTTGCAGGGAGAGAGGAAATAAGTCCCCAGGCTCCAAGGCTGACCGGGGTGGGGTCTCCGCAGGTAACTGTGCTGAGGGTCTGGGTACGAACTACCGAGGGCATGTGAACATCACCCGGTCAGGCATTGAGTGCCAGCTATGGAGGAGTCGCTACCCACATAAGCCTGAGTGAGTGAGGGGTCGGCCTTCCCACCATGGGCTGAGAACAGGGAGCAAGCGTACCTCAAGTTCAACAGCCTCCTGTTGGGCAATTTCCTCTTCCAGAATCAACTCCACTACCCATCCTGGGGCCGACCTACAGGAGAATTTCTGCCGCAACCCCGACAGCAGCACCACGGGACCCTGGTGCTACACTACAGACCCCACCGTGAGGAGGCAGGAATGCAGCATCCCTGTCTGTGGTAGGCTGGGGGCAGTGGGGCGACCCATGACCAAGCCCGGGGGCTTCATGGGGCCTGGCAGCCTGGGATGGGAACCAAGAATACTGGCTACCCAGGCACAGTGGCTCATGCCCGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACCTGAGGTCAGGGGTTTGAGACCAGCTGGGCCAACATGGCAAAACCCCGTCTCTACTAAAAATACAAAAATTGCCAGGCGTGGTGGTGGGCGCCTGTAATCCCAACTACTCTGGAGGCTGAGGCACGAGAATCGCTTGAACCCGGGAG
PRIMER_LEFT_EXPLAIN=considered 4464, overlap target 289, GC content failed 2, low tm 392, high tm 2999, ok 782
PRIMER_RIGHT_EXPLAIN=considered 4464, overlap target 289, GC content failed 40, low tm 325, high tm 3245, ok 565
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=1184.087291
PRIMER_LEFT_0_PENALTY=0.806671
PRIMER_RIGHT_0_PENALTY=1183.280619
PRIMER_LEFT_0_SEQUENCE=GTTGGGCAATTTCCTCTTCC
PRIMER_RIGHT_0_SEQUENCE=CTTGGTTCCCATCCCAGG
PRIMER_LEFT_0=278,20
PRIMER_RIGHT_0=515,18
PRIMER_LEFT_0_TM=60.807
PRIMER_RIGHT_0_TM=61.281
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=1180.000000
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=238
PRIMER_PAIR_0_PRODUCT_TM=82.9
=
SEQUENCE_ID=F2_X7
SEQUENCE_TARGET=298,319
SEQUENCE_TEMPLATE=GCAGCTCCAGGCAGCCACTACCAGTTGGTTACAGATGGCCTAGGAGGCCAAACCTGGTTACTATCTCTGGTTTATTATGTGCCAGACACTTATGCTGTATATTTTGTTTAATCCTCTCAACAAACCTGCAAAAGTGGCATTAGTAACCCCTTTAAAGGCAAACGGTCAGAAGCCCAGAGAGGTTAAGTAACCTGAGGTCACACAGGCAGAAAGCAGCAAGACCGGGGTTCACACCCCTGTCTGTTCCGGTCCATGTGTGGTCTCACTCACTCTGCTGCCTCCTTGCCCCTCACCCACCAGGCCAGGATCAAGTCACTGTAGCGATGACTCCACGCTCCGAAGGCTCCAGTGTGAATCTGTCACCTCCATTGGAGCAGTGTGTCCCTGATCGGGGGCAGCAGTACCAGGGGCGCCTGGCGGTGACCACACATGGGCTCCCCTGCCTGGCCTGGGCCAGCGCACAGGCCAAGGCCCTGAGCAAGCACCAGGACTTCAACTCAGCTGTGCAGCTGGTGGAGAACTTCTGCCGCAACCCAGACGGGGATGAGGAGGGCGTGTGGTGCTATGTGGCCGGGAAGCCTGGCGACTTTGGGTACTGCGACCTCAACTATTGTGGTGAGCTGCCTGGGTAGGGGGCCTGAGTTGCAGGGACAAATCCTAGTGGGAATAACAACAGCCGCTTCTGCTTATCGAACGCTTACCTCATTGAGTGCGCTCATTACAGCCTTACAGTAACCAGGTGGGGGGTAAGGTCCTGTGCCCATTTCACAGATAAGTACACTGAGGCCCCAGGAGGTTATTGCCTAGTAGCCCAACTGTGCATGCACGCTTAACCTCTGCACCAAATGGCCTCCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCC
PRIMER_LEFT_EXPLAIN=considered 6244, overlap target 289, GC content failed 60, low tm 973, high tm 3795, ok 1127
PRIMER_RIGHT_EXPLAIN=considered 5938, overlap target 289, GC content failed 51, low tm 580, high tm 3983, long poly-x seq 44, ok 991
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=369.200187
PRIMER_LEFT_0_PENALTY=224.254433
PRIMER_RIGHT_0_PENALTY=144.945754
PRIMER_LEFT_0_SEQUENCE=CTCTGCTGCCTCCTTGCC
PRIMER_RIGHT_0_SEQUENCE=CTCAGGCCCCCTACCCAG
PRIMER_LEFT_0=269,18
PRIMER_RIGHT_0=641,18
PRIMER_LEFT_0_TM=62.254
PRIMER_RIGHT_0_TM=62.946
PRIMER_LEFT_0_GC_PERCENT=66.667
PRIMER_RIGHT_0_GC_PERCENT=72.222
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=220.000000
PRIMER_RIGHT_0_POSITION_PENALTY=140.000000
PRIMER_LEFT_0_END_STABILITY=10.0000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=373
PRIMER_PAIR_0_PRODUCT_TM=84.2
=
SEQUENCE_ID=F2_X8
SEQUENCE_TARGET=298,133
SEQUENCE_TEMPLATE=GAGTTGCAGGGACAAATCCTAGTGGGAATAACAACAGCCGCTTCTGCTTATCGAACGCTTACCTCATTGAGTGCGCTCATTACAGCCTTACAGTAACCAGGTGGGGGGTAAGGTCCTGTGCCCATTTCACAGATAAGTACACTGAGGCCCCAGGAGGTTATTGCCTAGTAGCCCAACTGTGCATGCACGCTTAACCTCTGCACCAAATGGCCTCCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCCCGGCCGGTGCCTGGGTCCCAACAGAGGAGGCCGTGGAGGAGGAGACAGGAGATGGGCTGGATGAGGACTCAGACAGGGCCATCGAAGGGCGTACCGCCACCAGTGAGTACCAGACTTTCTTCAATCCGAGGACCTTTGGCTCGGGAGAGGCAGGTGAGGTAGTGGGCATCCGAGGGGATGCGGGGCTGCGGGGCTGGTGGCCAGGACTTGCCCCTCACTGCTTGGCTTGCTCTGCAGACTGTGGGCTGCGACCTCTGTTCGAGAAGAAGTCGCTGGAGGACAAAACCGAAAGAGAGCTCCTGGAATCCTACATCGACGGGCGCATTGTGGAGGGCTCGGATGCAGAGATCGGCATGTCACCTTGGTGTGTCCTGGAGCCCTGCGCTACCATTCACTCCTGGGGGCAGGTGTGCTGCTGGACCCCCACCCTCAGGCCCTGCCTGCAGGCCTGGG
PRIMER_LEFT_EXPLAIN=considered 4078, overlap target 289, GC content failed 87, low tm 484, high tm 2399, high end compl 1, long poly-x seq 44, ok 774
PRIMER_RIGHT_EXPLAIN=considered 4384, overlap target 289, GC content failed 97, low tm 244, high tm 3201, ok 553
PRIMER_PAIR_EXPLAIN=considered 14, high end compl 12, ok 2
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=706.658308
PRIMER_LEFT_0_PENALTY=683.278099
PRIMER_RIGHT_0_PENALTY=23.380210
PRIMER_LEFT_0_SEQUENCE=GGATGGGTGAGGAATGGC
PRIMER_RIGHT_0_SEQUENCE=CCTCGGATGCCCACTACCT
PRIMER_LEFT_0=246,18
PRIMER_RIGHT_0=450,19
PRIMER_LEFT_0_TM=61.278
PRIMER_RIGHT_0_TM=62.380
PRIMER_LEFT_0_GC_PERCENT=61.111
PRIMER_RIGHT_0_GC_PERCENT=63.158
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=680.000000
PRIMER_RIGHT_0_POSITION_PENALTY=20.000000
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=205
PRIMER_PAIR_0_PRODUCT_TM=83.0
=
SEQUENCE_ID=F2_X9
SEQUENCE_TARGET=298,131
SEQUENCE_TEMPLATE=CCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCCCGGCCGGTGCCTGGGTCCCAACAGAGGAGGCCGTGGAGGAGGAGACAGGAGATGGGCTGGATGAGGACTCAGACAGGGCCATCGAAGGGCGTACCGCCACCAGTGAGTACCAGACTTTCTTCAATCCGAGGACCTTTGGCTCGGGAGAGGCAGGTGAGGTAGTGGGCATCCGAGGGGATGCGGGGCTGCGGGGCTGGTGGCCAGGACTTGCCCCTCACTGCTTGGCTTGCTCTGCAGACTGTGGGCTGCGACCTCTGTTCGAGAAGAAGTCGCTGGAGGACAAAACCGAAAGAGAGCTCCTGGAATCCTACATCGACGGGCGCATTGTGGAGGGCTCGGATGCAGAGATCGGCATGTCACCTTGGTGTGTCCTGGAGCCCTGCGCTACCATTCACTCCTGGGGGCAGGTGTGCTGCTGGACCCCCACCCTCAGGCCCTGCCTGCAGGCCTGGGCTTTACAGATGACAACAGCTGAGCATCCAGGATCCCACCAACTCCACACAGCAGCCACATGAGATGGGTTGTTTACTTCTTTTTTTTTTGTTTCTTAGATGGAGTCTTGCTCTGTCACCTAGGCTGGAGTGCAGTGCTGCAATCTCGGCTCACTACCTCGATCTCAGCTCACTGCAACTTCTGCCTTCCGGGTTCAAACGATTCTCTTGCC
PRIMER_LEFT_EXPLAIN=considered 4212, overlap target 289, GC content failed 182, low tm 233, high tm 2939, long poly-x seq 22, ok 547
PRIMER_RIGHT_EXPLAIN=considered 4248, overlap target 289, GC content failed 80, low tm 415, high tm 2693, long poly-x seq 23, ok 748
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=166.167609
PRIMER_LEFT_0_PENALTY=2.845950
PRIMER_RIGHT_0_PENALTY=163.321659
PRIMER_LEFT_0_SEQUENCE=ACTGCTTGGCTTGCTCTGC
PRIMER_RIGHT_0_SEQUENCE=AATGGTAGCGCAGGGCTC
PRIMER_LEFT_0=279,19
PRIMER_RIGHT_0=454,18
PRIMER_LEFT_0_TM=61.846
PRIMER_RIGHT_0_TM=61.322
PRIMER_LEFT_0_GC_PERCENT=57.895
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=160.000000
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=176
PRIMER_PAIR_0_PRODUCT_TM=80.0
=
SEQUENCE_ID=F2_X10
SEQUENCE_TARGET=298,172
SEQUENCE_TEMPLATE=CCTGAGCCCAAGAGGCTGAGGCTTCAGTAAGCTGTGACTGTACCATTGCACTCCAGCCTGGGTGACAAGAGTGAGACCCTGTCTCAAAAATAAATAAATAAATAAATAAAAAGTGTGAGGCAGCCCCTCAGCATCACACGGAGGCTCCAGCCCCAAAGGCGGCCAGCCCAAGCTTGGATCTGGGCCCCGGAGGCAGCTCTGCCCAGCTGGGTTCTTAGACCTGGGATTGTTACTTCTAGGGCTGGTGTAGAGGCAGCCCCCTCATCCTCAGCTCCTAATGCTTCCTGCTGCCCCTCCCAGGCAGGTGATGCTTTTCCGGAAGAGTCCCCAGGAGCTGCTGTGTGGGGCCAGCCTCATCAGTGACCGCTGGGTCCTCACCGCCGCCCACTGCCTCCTGTACCCGCCCTGGGACAAGAACTTCACCGAGAATGACCTTCTGGTGCGCATTGGCAAGCACTCCCGCACAAGGTACAGAACTGGTGGCCCGTGGGTGTCTGGCAGGGGTCTGAGTCCTCCAAAGCGATCATGAGGGGCCTTGGTGGCTCCGGGACACATAGGATGTTCTGTATACCCCCCAGAATATAACATCCCAGCAGTCTCTGCTGGAAAGCCATTTGGTCACGTCCTGACTGAGGCTTGGAGCGCGGGGAGAATCCGTCTGTCTCTGGTCCCTCCAACACTAGGATATAGCCCATGTGGGAGTCTCTGAAAATAGAGTCTGTCTGGACTAGGGCGTGCAGCCTGTGCCCCTGTCCCCGTCCTCCAGGC
PRIMER_LEFT_EXPLAIN=considered 4774, overlap target 289, GC content failed 210, low tm 485, high tm 3153, ok 637
PRIMER_RIGHT_EXPLAIN=considered 4621, overlap target 289, GC content failed 8, low tm 474, high tm 3110, long poly-x seq 22, ok 718
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=105.472156
PRIMER_LEFT_0_PENALTY=101.889081
PRIMER_RIGHT_0_PENALTY=3.583075
PRIMER_LEFT_0_SEQUENCE=CTAATGCTTCCTGCTGCCC
PRIMER_RIGHT_0_SEQUENCE=ACGGGCCACCAGTTCTGT
PRIMER_LEFT_0=274,19
PRIMER_RIGHT_0=487,18
PRIMER_LEFT_0_TM=60.889
PRIMER_RIGHT_0_TM=61.583
PRIMER_LEFT_0_GC_PERCENT=57.895
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=100.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=11.2000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=214
PRIMER_PAIR_0_PRODUCT_TM=82.6
=
SEQUENCE_ID=F2_X11
SEQUENCE_TARGET=298,178
SEQUENCE_TEMPLATE=TGGTCCCTCCAACACTAGGATATAGCCCATGTGGGAGTCTCTGAAAATAGAGTCTGTCTGGACTAGGGCGTGCAGCCTGTGCCCCTGTCCCCGTCCTCCAGGCTGTCTGACTCCAAAGCCCTGCACGGCTTTAGGCCCAGGAAGAAACACCCAGGGGGCTGCCATGGCAGGAACCAGCCCTATCCCCTCCCTGGTGGCCTGCAGGACACACTGTCTCCCAGAACCCCAAGGGCAGGCAGTTTCCTGCTCCTTGCTGGGTGAACCTGCAGCTTCTCCATTTCTTTCTTGGGGTCTCTGCAGGTACGAGCGAAACATTGAAAAGATATCCATGTTGGAAAAGATCTACATCCACCCCAGGTACAACTGGCGGGAGAACCTGGACCGGGACATTGCCCTGATGAAGCTGAAGAAGCCTGTTGCCTTCAGTGACTACATTCACCCTGTGTGTCTGCCCGACAGGGAGACGGCAGCCAGGTGGGCCACCAGATGCTTGTTAGCTGAGGGGCAGAAGCCAAGTTCTGGGCCTGGCTCTGATACCAAGTAGCCTTGCAAGAGCCCCTTTCCCTTTTCCAGGCCTCGGTTTCTTGGAGTGAACCCAAAAGTTCTTTTCAGTACTGGCGTTTTATTTTTTATTTATATTTATTTATTTACTGACGGAGTTCCACTCTTGTCTCCCAGGCTGGAGTGTAGTTGTGCGATCTTGGCTCACTGCAACCCCACCTCCTGGGTTCAAGCGACTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACA
PRIMER_LEFT_EXPLAIN=considered 4834, overlap target 289, low tm 620, high tm 3010, ok 915
PRIMER_RIGHT_EXPLAIN=considered 4762, overlap target 289, GC content failed 176, low tm 739, high tm 2528, long poly-x seq 13, ok 1017
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=4.703189
PRIMER_LEFT_0_PENALTY=0.594713
PRIMER_RIGHT_0_PENALTY=4.108476
PRIMER_LEFT_0_SEQUENCE=TTCTTTCTTGGGGTCTCTGC
PRIMER_RIGHT_0_SEQUENCE=ACAAGCATCTGGTGGCCC
PRIMER_LEFT_0=278,20
PRIMER_RIGHT_0=493,18
PRIMER_LEFT_0_TM=59.405
PRIMER_RIGHT_0_TM=62.108
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=12.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=216
PRIMER_PAIR_0_PRODUCT_TM=79.0
=
SEQUENCE_ID=F2_X12
SEQUENCE_TARGET=298,186
SEQUENCE_TEMPLATE=CCACCTCCTGGGTTCAAGCGACTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACAGGCTAATTTTTGTATTTTTAGTAGAGACTGGTGGGTTTCACCGTGTCGGCCAGGTTGGTCTCGAACCCCTGACCTCAAGTGATTCACCCGCCTCGGCCTCCCAAAGTGCCGAGACCACAGGCGTGAACGTCTGTGCCCAGCCAGCTCTGGCGTTTTAGATTCTGGTCTCTAAGAAATGGCGTTGGGGCCAGGCGGCTCCTGTGGGGGTTGGCTCTCACTAGGCCCTTCTTCCTTCCCCAAAGCTTGCTCCAGGCTGGATACAAGGGGCGGGTGACAGGCTGGGGCAACCTGAAGGAGACGTGGACAGCCAACGTTGGTAAGGGGCAGCCCAGTGTCCTGCAGGTGGTGAACCTGCCCATTGTGGAGCGGCCGGTCTGCAAGGACTCCACCCGGATCCGCATCACTGACAACATGTTCTGTGCTGGCAAGTCTGTGCAGGGCGGGCTGAGGGAACAGTGGGGCCCAAGCTGGGAGAACTGAGTTGTGCCTGGGTTCAAGCCATGTGACTTTGAGCAAGTTGCCTAACCTCTTGGTGGCTCAGTTTCTTCCTCTGTAAAATGGAGGTAAAAGTCTCTATCCCATAAGGTTATGGGAGGGTTAAATGAAGTAGTATATATTAATGTACTTGGCATAGTATCAGTCACCAGTGAGCTCAGATAGCAGCAAGAGGCTGCGGGTAGGGAAATGCCATTCATTCAGTCACTCAGCAAATATTTATTGAGCG
PRIMER_LEFT_EXPLAIN=considered 4914, overlap target 289, GC content failed 56, low tm 524, high tm 3341, ok 704
PRIMER_RIGHT_EXPLAIN=considered 4914, overlap target 289, GC content failed 83, low tm 976, high tm 2660, ok 906
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=286.895207
PRIMER_LEFT_0_PENALTY=2.027407
PRIMER_RIGHT_0_PENALTY=284.867800
PRIMER_LEFT_0_SEQUENCE=CCCTTCTTCCTTCCCCAA
PRIMER_RIGHT_0_SEQUENCE=CACTGTTCCCTCAGCCCG
PRIMER_LEFT_0=280,18
PRIMER_RIGHT_0=515,18
PRIMER_LEFT_0_TM=59.973
PRIMER_RIGHT_0_TM=62.868
PRIMER_LEFT_0_GC_PERCENT=55.556
PRIMER_RIGHT_0_GC_PERCENT=66.667
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=280.000000
PRIMER_LEFT_0_END_STABILITY=10.0000
PRIMER_RIGHT_0_END_STABILITY=12.9000
PRIMER_PAIR_0_COMPL_ANY=2.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=236
PRIMER_PAIR_0_PRODUCT_TM=82.6
=
SEQUENCE_ID=F2_X13
SEQUENCE_TARGET=298,75
SEQUENCE_TEMPLATE=AACTTTTTAAAAGCATTATTCTGCCTGTTGGGTGGAGAATAGACTGTAGGTGGGCAAAGAATGAAGGAAACTAGTGGGTTCAGGAGCTCGAGCTAGAAGTGGTGAGAAGGGTTTGGATTTGGGGTCTATGCTGAAGGTAGAGCCGACAAGATTTGCTAGGATTGGATGTGTAGGGTGAGGAAGTGGGGACAGCAAGAATGACTGGAGGGGTAAGTGGACTCTCACCAGCTGTGTCTCGTGAAGGGGCGTGGCTGGGCTATGAGCTATGCTCCTGAGCACAGACGGCTGTTCTCTTTCAAGGTTACAAGCCTGATGAAGGGAAACGAGGGGATGCCTGTGAAGGTGACAGTGGGGGACCCTTTGTCATGAAGGTAAGCTTCTCTAAAGCCCAGGGCCTGGTGAACACATCTTCTGGGGGTGGGGAGAAACTCTAGTATCTAGAAACAGTTGCCTGGCAGAGGAATACTGATGTGACCTTGAACTTGACTCTATTGGAAACCTCATCTTTCTTCTTCAGAGCCCCTTTAACAACCGCTGGTATCAAATGGGCATCGTCTCATGGGGTGAAGGCTGTGACCGGGATGGGAAATATGGCTTCTACACACATGTGTTCCGCCTGAAGAAGTGGATACAGAAGGTCATTGATCAGTTTGGAGAGTAGGGGGCCACTC
PRIMER_LEFT_EXPLAIN=considered 3804, overlap target 289, GC content failed 12, low tm 652, high tm 1841, ok 1010
PRIMER_RIGHT_EXPLAIN=considered 3804, overlap target 289, low tm 873, high tm 1715, high end compl 5, ok 922
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=62.821994
PRIMER_LEFT_0_PENALTY=0.838952
PRIMER_RIGHT_0_PENALTY=61.983042
PRIMER_LEFT_0_SEQUENCE=CAGACGGCTGTTCTCTTTCA
PRIMER_RIGHT_0_SEQUENCE=GCCCTGGGCTTTAGAGAAG
PRIMER_LEFT_0=278,20
PRIMER_RIGHT_0=394,19
PRIMER_LEFT_0_TM=59.161
PRIMER_RIGHT_0_TM=59.017
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=60.000000
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=117
PRIMER_PAIR_0_PRODUCT_TM=76.5
=
SEQUENCE_ID=F2_X14
SEQUENCE_TARGET=298,148
SEQUENCE_TEMPLATE=ACTCTCACCAGCTGTGTCTCGTGAAGGGGCGTGGCTGGGCTATGAGCTATGCTCCTGAGCACAGACGGCTGTTCTCTTTCAAGGTTACAAGCCTGATGAAGGGAAACGAGGGGATGCCTGTGAAGGTGACAGTGGGGGACCCTTTGTCATGAAGGTAAGCTTCTCTAAAGCCCAGGGCCTGGTGAACACATCTTCTGGGGGTGGGGAGAAACTCTAGTATCTAGAAACAGTTGCCTGGCAGAGGAATACTGATGTGACCTTGAACTTGACTCTATTGGAAACCTCATCTTTCTTCTTCAGAGCCCCTTTAACAACCGCTGGTATCAAATGGGCATCGTCTCATGGGGTGAAGGCTGTGACCGGGATGGGAAATATGGCTTCTACACACATGTGTTCCGCCTGAAGAAGTGGATACAGAAGGTCATTGATCAGTTTGGAGAGTAGGGGGCCACTCATATTCTGGGCTCCTGGAACCAATCCCGTGAAAGAATTATTTTTGTGTTTCTAAAACTATGGTTCCCAATAAAAGTGACTCTCAGCGAGCCTCAATGCTCCCAGTGCTATTCATGGGCAGCTCTCTGGGCTCAGGAAGAGCCAGTAATACTACTGGATAAAGAAGACTTAAGAATCCACCACCTGGTGCACGCTGGTAGTCCGAGCACTCGGGAGGCTGAGGTGGGAGGAT
PRIMER_LEFT_EXPLAIN=considered 4475, overlap target 230, low tm 988, high tm 2162, ok 1095
PRIMER_RIGHT_EXPLAIN=considered 3944, overlap target 289, GC content failed 74, low tm 898, high tm 1848, high end compl 2, ok 833
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=24.449698
PRIMER_LEFT_0_PENALTY=4.108177
PRIMER_RIGHT_0_PENALTY=20.341522
PRIMER_LEFT_0_SEQUENCE=TTGGAAACCTCATCTTTCTTCTTC
PRIMER_RIGHT_0_SEQUENCE=GAGCCCAGAATATGAGTGGC
PRIMER_LEFT_0=274,24
PRIMER_RIGHT_0=466,20
PRIMER_LEFT_0_TM=60.108
PRIMER_RIGHT_0_TM=59.658
PRIMER_LEFT_0_GC_PERCENT=37.500
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=20.000000
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=193
PRIMER_PAIR_0_PRODUCT_TM=77.0
=
SEQUENCE_ID=F2R_X1
SEQUENCE_TARGET=235,90
SEQUENCE_TEMPLATE=CTTCCTCGCTCGCGCCCGCGCGACCGCGCGCCCCAGTCCCGCCCCGCCCCGCTAACCGCCCCAGACACAGCGCTCGCCGAGGGTCGCTTGGACCCTGATCTTACCCGTGGGCACCCTGCGCTCTGCCTGCCGCGAAGACCGGCTCCCCGACCCGCAGAAGTCAGGAGAGAGGGTGAAGCGGAGCAGCCCGAGGCGGGGCAGCCTCCCGGAGCAGCGCCGCGCAGAGCCCGGGACAATGGGGCCGCGGCGGCTGCTGCTGGTGGCCGCCTGCTTCAGTCTGTGCGGCCCGCTGTTGTCTGCCCGCACCCGGGCCCGCAGGCCAGGTGAGA
PRIMER_LEFT_EXPLAIN=considered 2940, GC content failed 829, low tm 31, high tm 1984, ok 96
PRIMER_RIGHT_EXPLAIN=considered 951, overlap target 226, GC content failed 209, high tm 510, ok 6
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=57988.526397
PRIMER_LEFT_0_PENALTY=984.110493
PRIMER_RIGHT_0_PENALTY=57004.415904
PRIMER_LEFT_0_SEQUENCE=GAGGGTGAAGCGGAGCAG
PRIMER_RIGHT_0_SEQUENCE=GCCGCACAGACTGAAGCA
PRIMER_LEFT_0=168,18
PRIMER_RIGHT_0=285,18
PRIMER_LEFT_0_TM=62.110
PRIMER_RIGHT_0_TM=62.416
PRIMER_LEFT_0_GC_PERCENT=66.667
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=980.000000
PRIMER_RIGHT_0_POSITION_PENALTY=57000.000000
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=118
PRIMER_PAIR_0_PRODUCT_TM=86.1
=
SEQUENCE_ID=F2R_X2-seg1
SEQUENCE_TARGET=4,702
SEQUENCE_TEMPLATE=TTTCAGAATCAAAAGCAACAAATGCCACCTTAGATCCCCGGTCATTTCTTCTCAGGAACCCCAATGATAAATATGAACCATTTTGGGAGGATGAGGAGAAAAATGAAAGTGGGTTAACTGAATACAGATTAGTCTCCATCAATAAAAGCAGTCCTCTTCAAAAACAACTTCCTGCATTCATCTCAGAAGATGCCTCCGGATATTTGACCAGCTCCTGGCTGACACTCTTTGTCCCATCTGTGTACACCGGAGTGTTTGTAGTCAGCCTCCCACTAAACATCATGGCCATCGTTGTGTTCATCCTGAAAATGAAGGTCAAGAAGCCGGCGGTGGTGTACATGCTGCACCTGGCCACGGCAGATGTGCTGTTTGTGTCTGTGCTCCCCTTTAAGATCAGCTATTACTTTTCCGGCAGTGATTGGCAGTTTGGGTCTGAATTGTGTCGCTTCGTCACTGCAGCATTTTACTGTAACATGTACGCCTCTATCTTGCTCATGACAGTCATAAGCATTGACCGGTTTCTGGCTGTGGTGTATCCCATGCAGTCCCTCTCCTGGCGTACTCTGGGAAGGGCTTCCTTCACTTGTCTGGCCATCTGGGCTTTGGCCATCGCAGGGGTAGTGCCTCTCGTCCTCAAGGAGCAAACCATCCAGGTGCCCGGGCTCAACATCACTACCTGTCATGATGTGCTCAATGAAACCCTGCT
PRIMER_LEFT_EXPLAIN=considered 6710, GC content failed 4, low tm 1539, high tm 3376, high repeat similarity 1, ok 1790
PRIMER_RIGHT_EXPLAIN=considered 6710, GC content failed 4, low tm 1540, high tm 3376, high repeat similarity 2, ok 1788
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=39005.267605
PRIMER_LEFT_0_PENALTY=19005.004751
PRIMER_RIGHT_0_PENALTY=20000.262854
PRIMER_LEFT_0_SEQUENCE=TTTCAGAATCAAAAGCAACAAAT
PRIMER_RIGHT_0_SEQUENCE=AGCAGGGTTTCATTGAGCAC
PRIMER_LEFT_0=0,23
PRIMER_RIGHT_0=705,20
PRIMER_LEFT_0_TM=57.995
PRIMER_RIGHT_0_TM=60.263
PRIMER_LEFT_0_GC_PERCENT=26.087
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=19000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=20000.000000
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=706
PRIMER_PAIR_0_PRODUCT_TM=78.9
=
SEQUENCE_ID=F2R_X2-seg2
SEQUENCE_TARGET=0,514
SEQUENCE_TEMPLATE=TGATGTGCTCAATGAAACCCTGCTCGAAGGCTACTATGCCTACTACTTCTCAGCCTTCTCTGCTGTCTTCTTTTTTGTGCCGCTGATCATTTCCACGGTCTGTTATGTGTCTATCATTCGATGTCTTAGCTCTTCCGCAGTTGCCAACCGCAGCAAGAAGTCCCGGGCTTTGTTCCTGTCAGCTGCTGTTTTCTGCATCTTCATCATTTGCTTCGGACCCACAAACGTCCTCCTGATTGCGCATTACTCATTCCTTTCTCACACTTCCACCACAGAGGCTGCCTACTTTGCCTACCTCCTCTGTGTCTGTGTCAGCAGCATAAGCTCGTGCATCGACCCCCTAATTTACTATTACGCTTCCTCTGAGTGCCAGAGGTACGTCTACAGTATCTTATGCTGCAAAGAAAGTTCCGATCCCAGCAGTTATAACAGCAGTGGGCAGTTGATGGCAAGTAAAATGGATACCTGCTCTAGTAACCTGAATAACAGCATATACAAAAAGCTGTTAACTTAGGAAAAGGGACTGCTGGGAGGTTAAAAAGAAAAGTTTATAAAAGTGAATAACCTGAGGATTCTATTAGTCCCCACCCAAACTTTATTGATTCACCTCCTAAAACAACAGATGTACGACTTGCATACCTGCTTTTTATGGGAGCTGTCAAGCATGTATTTTTGTCAATTACCAGAAAGATAACAGGACGAGATGACGGTGTTATTCCAAGGGAATATTGCCAATGCTACAGTAATAAA
PRIMER_LEFT_EXPLAIN=considered 4999, overlap target 227, low tm 1458, high tm 1982, long poly-x seq 22, ok 1310
PRIMER_RIGHT_EXPLAIN=considered 6997, GC content failed 109, low tm 2483, high tm 2360, long poly-x seq 22, ok 2023
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=20001.710366
PRIMER_LEFT_0_PENALTY=20000.498267
PRIMER_RIGHT_0_PENALTY=1.212099
PRIMER_LEFT_0_SEQUENCE=TGATGTGCTCAATGAAACCC
PRIMER_RIGHT_0_SEQUENCE=CTCCCAGCAGTCCCTTTTC
PRIMER_LEFT_0=0,20
PRIMER_RIGHT_0=532,19
PRIMER_LEFT_0_TM=59.502
PRIMER_RIGHT_0_TM=59.788
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=20000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=533
PRIMER_PAIR_0_PRODUCT_TM=78.2
=
SEQUENCE_ID=F3_X1
SEQUENCE_TARGET=298,104
SEQUENCE_TEMPLATE=ACCGGGAGGAGGCGGGGCAGGGGTGTGGACTCGCCGGGGGCCGCCCACCGCGACGGCAAGTGACCCGGGCCGGGGGCGGGGAGTCGGGAGGAGCGGCGGGGGCGGGCGCCGGGGGCGGGCAGAGGCGCGGGAGAGCGCGCCGCCGGCCCTTTATAGCGCGCGGGGCACCGGCTCCCCAAGACTGCGAGCTCCCCGCACCCCCTCGCACTCCCTCTGGCCGGCCCAGGGCGCCTTCAGCCCAACCTCCCCAGCCCCACGGGCGCCACGGAACCCGCTCGATCTCGCCGCCAACTGGTAGACATGGAGACCCCTGCCTGGCCCCGGGTCCCGCGCCCCGAGACCGCCGTCGCTCGGACGCTCCTGCTCGGCTGGGTCTTCGCCCAGGTGGCCGGCGCTTCAGGTGAGTGGCACCAGCCCCTGGAAGCCCGGGGCGCGCCACACGCAGGAGGGAGGCGACAGTCCTGGCTGGCAGCGGGCTCGCCCTGGTTCCCCGGGGCGCCCATGTTGTCCCCCGCGCCTACGGGACTCGGCTGCGCTCACCCAGCCCGGCTTGAATGAACCGAGTCCGTCGGGCGCCGGCGGGAGTTGCAGGGAGGGAGTTGGCGCCCCAGACCCCGCTGCCCCTTCCGCTGGAGAGTTTTGCTCGGGGTGTCCGAGTAATTGGACTGTTGTTGCATAAGCGGACTTTTAGCTCCCGCTT
PRIMER_LEFT_EXPLAIN=considered 4094, overlap target 289, GC content failed 1473, low tm 23, high tm 2263, ok 46
PRIMER_RIGHT_EXPLAIN=considered 4094, overlap target 289, GC content failed 716, low tm 101, high tm 2783, ok 205
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=808.329006
PRIMER_LEFT_0_PENALTY=4.915955
PRIMER_RIGHT_0_PENALTY=803.413051
PRIMER_LEFT_0_SEQUENCE=CTCGCCGCCAACTGGTAG
PRIMER_RIGHT_0_SEQUENCE=ACTGTCGCCTCCCTCCTG
PRIMER_LEFT_0=280,18
PRIMER_RIGHT_0=459,18
PRIMER_LEFT_0_TM=62.916
PRIMER_RIGHT_0_TM=61.413
PRIMER_LEFT_0_GC_PERCENT=66.667
PRIMER_RIGHT_0_GC_PERCENT=66.667
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=800.000000
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=180
PRIMER_PAIR_0_PRODUCT_TM=86.9
=
SEQUENCE_ID=F3_X2
SEQUENCE_TARGET=298,116
SEQUENCE_TEMPLATE=CTCTGGGCTCTTCCCGGTCAAGTTATAACAAGGCTTTGCCCATGAATAATTTCAAACGAAAATGTCAAGATCCTTGCCGGTGTCCTGGGATTACAAGGTGAATCTTGTCATGAAGAAATTCTAGGTCTAGAAAAAATTTGAAGATTCTTTTTCTCTTGATAATTCACTAATGAAGCTTTTGTGGTTGAAAAATAAAAAGTGAGGTTTATGGTGATGTCAGGTGGGAAGGTGTTTTATACATCAATACATTCGAGTGCTCTGAAGTGCATGTAATAATAGCTGTTTCTCTGTTGTTTAAAGGCACTACAAATACTGTGGCAGCATATAATTTAACTTGGAAATCAACTAATTTCAAGACAATTTTGGAGTGGGAACCCAAACCCGTCAATCAAGTCTACACTGTTCAAATAAGGTAAGCTGGGTACAGAAAAAGAAAATTAAGGTCTTTGATGTTTCTACTGTCCTATGCTGAACAAGAATGTCTTTAAAGCTGATTACTGGATGAAATTATTTAACAGATGACGAAGAAGAAGGGATTCTTGGCAATTCGCTGGCCGGTGTCATACTCTATTAGGCCTGCAACATTTCCAGACCTTAAACTGATAGAACATTTTAATTGTTTTAATTGTTTTTGGAAATGATGGGAGAGTTCCTAAGTGGAGTATAAACTGTGGAGAGATGAACCATCTTGAGTAGGCACTGAAGTGTGCTT
PRIMER_LEFT_EXPLAIN=considered 4082, overlap target 289, GC content failed 83, low tm 1878, high tm 779, high repeat similarity 1, long poly-x seq 20, ok 1032
PRIMER_RIGHT_EXPLAIN=considered 4214, overlap target 289, GC content failed 232, low tm 1968, high tm 682, ok 1043
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=113.663477
PRIMER_LEFT_0_PENALTY=109.223369
PRIMER_RIGHT_0_PENALTY=4.440107
PRIMER_LEFT_0_SEQUENCE=CATGTAATAATAGCTGTTTCTCTGTTG
PRIMER_RIGHT_0_SEQUENCE=TTTTCTTTTTCTGTACCCAGCTT
PRIMER_LEFT_0=266,27
PRIMER_RIGHT_0=436,23
PRIMER_LEFT_0_TM=57.777
PRIMER_RIGHT_0_TM=58.560
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=34.783
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=100.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=171
PRIMER_PAIR_0_PRODUCT_TM=70.8
=
SEQUENCE_ID=F3_X3
SEQUENCE_TARGET=298,204
SEQUENCE_TEMPLATE=TGAGGACTCAAACATGCATCCCAAGTGCTCGGTGTTGCTAGGTATGATGGCTCACACCTGTACATTCAGCACTTTGGGAGGCCGAAGCAGAAGGATCAGCCTGGGCAACATAGCAGGACCCCATCTCTACAAAACAATGTTTAAAAAAAAGCAAAGTGCTCAGCACAGTGACTGCATCATTAGGATTGATTGTAGGGCTCCTGATGTTAGCACAGAACACCACAGCCAGGAAGCAGTCTATCTTGTTGGGTGCAAATTGTAACATTCCATTTATGTTTCTTCCTTCTTTTCTTTCTTTAGCACTAAGTCAGGAGATTGGAAAAGCAAATGCTTTTACACAACAGACACAGAGTGTGACCTCACCGACGAGATTGTGAAGGATGTGAAGCAGACGTACTTGGCACGGGTCTTCTCCTACCCGGCAGGGAATGTGGAGAGCACCGGTTCTGCTGGGGAGCCTCTGTATGAGAACTCCCCAGAGTTCACACCTTACCTGGAGAGTAAGTGGCTTGGGCTGTAATACCGTTCATTCTTGTTAGAAACGTCTGAACATTCTCGTGATCTTGTGCCTTTAGGGGCTACAAAATTAAAAATATTTATTCTTTTTTTCTCAGAAACTGGTATGTATCACAGCCCTCTTCACACATTCCAGATGTGGTAGGAGGTTCACAGAATGTGAACTTTTGGAGCTGATGACAGTGTCATCAAGTAACTTTCTCCCCCAGTCTGTCCCCAGACCCTGTTACTGTCCTCAGTAAGCGGCTGAATGTGTGTTGGGAGAGGGCGGGCCAGGGAAGCGG
PRIMER_LEFT_EXPLAIN=considered 4965, overlap target 289, GC content failed 54, low tm 1212, high tm 2106, high repeat similarity 28, long poly-x seq 22, ok 1254
PRIMER_RIGHT_EXPLAIN=considered 5005, overlap target 289, GC content failed 185, low tm 1231, high tm 1945, long poly-x seq 11, ok 1344
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=209.077263
PRIMER_LEFT_0_PENALTY=206.472471
PRIMER_RIGHT_0_PENALTY=2.604792
PRIMER_LEFT_0_SEQUENCE=TTCCATTTATGTTTCTTCCTTCTT
PRIMER_RIGHT_0_SEQUENCE=TATTACAGCCCAAGCCACTT
PRIMER_LEFT_0=264,24
PRIMER_RIGHT_0=521,20
PRIMER_LEFT_0_TM=57.528
PRIMER_RIGHT_0_TM=57.395
PRIMER_LEFT_0_GC_PERCENT=29.167
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=200.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=258
PRIMER_PAIR_0_PRODUCT_TM=77.3
=
SEQUENCE_ID=F3_X4
SEQUENCE_TARGET=298,183
SEQUENCE_TEMPLATE=TGGTTAAATAAAATATATTATTAAAATTAATTTCACCTGTTCCTTTTTACTTTTTCTAATGTGACTACTAGAAAACTTAAAATGACATCTGAGGCTCCATTGTCTTCCCCTTGGGCCAGCACTACCACAGAATGTCTTAGGATTCAGCTCCAGGCCGCCACGCCTGCTTCTTTCAGGGAGCTGGTTCTATGCACATGTTTTATATGAGAGATAATTAAGTTGTCAATTGTGATAACAAAACAGGATTTGACTTTGTACAGAATTCTTTGGTTCCAACCAAGCTCATTTCCTTTGTTTCAGCAAACCTCGGACAGCCAACAATTCAGAGTTTTGAACAGGTGGGAACAAAAGTGAATGTGACCGTAGAAGATGAACGGACTTTAGTCAGAAGGAACAACACTTTCCTAAGCCTCCGGGATGTTTTTGGCAAGGACTTAATTTATACACTTTATTATTGGAAATCTTCAAGTTCAGGAAAGGTGAGCATTTTTTAATTTGTTTTTATGACCTGTTTTAAATTGTGAATACTTGGTTTTACAACCCATTTCTTCCCCAATTCAAAAATAGCAGAACAGAGTTGTTGAGAAGGTGATGGAGTAGAAGGGGGAGCGCGCACTGTGGGGAGGGGTGGACAACAGGCCTGGTCCTACCTGTGACTCTGCACTACCCTGTGACTCTGGCAGGGCCCCCTCGGAGACCCAGGTTCCTCAGCCAACCGGCTGGATCAGGTCATCTCTAAAGGTCCCGCCACGCTCACATTTCTCCCTCTATTGAGGATC
PRIMER_LEFT_EXPLAIN=considered 4884, overlap target 289, GC content failed 320, low tm 1911, high tm 1322, high end compl 6, ok 1036
PRIMER_RIGHT_EXPLAIN=considered 4770, overlap target 289, GC content failed 176, low tm 1330, high tm 1913, long poly-x seq 21, ok 1041
PRIMER_PAIR_EXPLAIN=considered 36, high end compl 33, ok 3
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=330.158482
PRIMER_LEFT_0_PENALTY=2.108929
PRIMER_RIGHT_0_PENALTY=328.049552
PRIMER_LEFT_0_SEQUENCE=CCAAGCTCATTTCCTTTGTTTC
PRIMER_RIGHT_0_SEQUENCE=TCACAATTTAAAACAGGTCATAAAAAC
PRIMER_LEFT_0=276,22
PRIMER_RIGHT_0=523,27
PRIMER_LEFT_0_TM=60.109
PRIMER_RIGHT_0_TM=58.950
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=25.926
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=320.000000
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=248
PRIMER_PAIR_0_PRODUCT_TM=72.9
=
SEQUENCE_ID=F3_X5
SEQUENCE_TARGET=298,164
SEQUENCE_TEMPLATE=CAAAATTTGTTTTTGGTTCAATGCATAATACTCCCTTCCTTTTTCTTTTACTGCAGATATCTTCTAAAGGGGCTCAATAGGGTTCAATATGCCTAAATTGGATCTTCTCAGTCTTGGAAAAGGCATTTTTAGCAGTGATCAAGGGAAACTGATTAGCGAAGTCACTTCTAATCCTTCACGTGTCAGCTGTGTTCTTGTAGGCTTTGCTTAGAACCTAGGTTTTTACTTCCACAGTGACTTAATAAAGGGGAAAGAATTGACTCAGAGCCCAGATGAATTAAGAACTCTATCTTTTTACAGAAAACAGCCAAAACAAACACTAATGAGTTTTTGATTGATGTGGATAAAGGAGAAAACTACTGTTTCAGTGTTCAAGCAGTGATTCCCTCCCGAACAGTTAACCGGAAGAGTACAGACAGCCCGGTAGAGTGTATGGGCCAGGAGAAAGGGGAATTCAGAGGTGAGTGGCTCTGCCAGCCATTTGCCTGGGGGTATGGGTGCTGTGGGTGACTTCTGGAGGAGTAGCTCCACCCTCAGGGCTGGGATATACTTCCTTGGTTAAATATTCAGGAAAACAAACTGCCTGGAGGTTTTTTGTTGTTATTTGTTTGTTTTGGTTTTGATTTTGCTTTGGTACAAAAAAGATTTTGGACATTTAGAAATGTTTCTGTGTTGATTGTGCCCTTGTATTAGCAGGTGTTTTCTTGAGCACCTGTCATGTGCTAAGCCCTCTGCTGAGCACTGGATACACAAACTGTGT
PRIMER_LEFT_EXPLAIN=considered 4694, overlap target 289, GC content failed 4, low tm 2035, high tm 844, ok 1522
PRIMER_RIGHT_EXPLAIN=considered 4397, overlap target 289, GC content failed 28, low tm 1323, high tm 1565, high end compl 4, long poly-x seq 44, ok 1144
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=194.838849
PRIMER_LEFT_0_PENALTY=69.931898
PRIMER_RIGHT_0_PENALTY=124.906952
PRIMER_LEFT_0_SEQUENCE=CCAGATGAATTAAGAACTCTATCTTTT
PRIMER_RIGHT_0_SEQUENCE=GGCAAATGGCTGGCAGAG
PRIMER_LEFT_0=268,27
PRIMER_RIGHT_0=485,18
PRIMER_LEFT_0_TM=57.068
PRIMER_RIGHT_0_TM=62.907
PRIMER_LEFT_0_GC_PERCENT=29.630
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=7.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=60.000000
PRIMER_RIGHT_0_POSITION_PENALTY=120.000000
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=218
PRIMER_PAIR_0_PRODUCT_TM=75.2
=
SEQUENCE_ID=F3_X6
SEQUENCE_TARGET=298,141
SEQUENCE_TEMPLATE=CAGCATCGTTTTAGGCACTTCTTTCCAGCCATTGTTCTTACCTCCAACTACATATCTTTTCTGGACTGTGCATTATTCAGTTTATTAAATGCCCATTAAATGTGTTTAGCCATTGTCAATTACTCTGAAACGTTCAGGTTTTGACAAATTCTTTCCTAATGTAAGTGTGGTGGAAAGAGTGAAAGAAAGTCAAATTGCACAAAAATAGGATGGTGTAATTTGGGGTTATGCCGTCAATTTTGTCCACTGATAAATGGGATTTGAGCTCTCCAAGTTGACTAGATGCCCTTTATTTTTCAGAAATATTCTACATCATTGGAGCTGTGGTATTTGTGGTCATCATCCTTGTCATCATCCTGGCTATATCTCTACACAAGTGTAGAAAGGCAGGAGTGGGGCAGAGCTGGAAGGAGAACTCCCCACTGAATGTTTCATAAAGGAAGCACTGTTGGAGCTACTGCAAATGCTATATTGCACTGTGACCGAGAACTTTTAAGAGGATAGAATACATGGAAACGCAAATGAGTATTTCGGAGCATGAAGACCCTGGAGTTCAAAAAACTCTTGATATGACCTGTTATTACCATTAGCATTCTGGTTTTGACATCAGCATTAGTCACTTTGAAATGTAACGAATGGTACTACAACCAATTCCAAGTTTTAATTTTTAACACCATGGCACCTTTTGCACATAACATGCTTTAGATTATATATTCCGCACTCAAGGAGTAACCAGG
PRIMER_LEFT_EXPLAIN=considered 4464, overlap target 289, GC content failed 57, low tm 1831, high tm 878, ok 1409
PRIMER_RIGHT_EXPLAIN=considered 4311, overlap target 289, GC content failed 51, low tm 1766, high tm 895, long poly-x seq 22, ok 1288
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=9.218677
PRIMER_LEFT_0_PENALTY=5.991812
PRIMER_RIGHT_0_PENALTY=3.226865
PRIMER_LEFT_0_SEQUENCE=TTGACTAGATGCCCTTTATTTTTC
PRIMER_RIGHT_0_SEQUENCE=CAGTAGCTCCAACAGTGCTTC
PRIMER_LEFT_0=274,24
PRIMER_RIGHT_0=459,21
PRIMER_LEFT_0_TM=58.008
PRIMER_RIGHT_0_TM=57.773
PRIMER_LEFT_0_GC_PERCENT=33.333
PRIMER_RIGHT_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=186
PRIMER_PAIR_0_PRODUCT_TM=74.5
=
SEQUENCE_ID=F5_X10
SEQUENCE_TARGET=59,219
SEQUENCE_TEMPLATE=TTATTTATTATCATGAAATAACTTTGCAAATGAAAACAATTTTGAATATATTTTCTTTCAGGCAGGAACAACACCATGATCAGAGCAGTTCAACCAGGGGAAACCTATACTTATAAGTGGAACATCTTAGAGTTTGATGAACCCACAGAAAATGATGCCCAGTGCTTAACAAGACCATACTACAGTGACGTGGACATCATGAGAGACATCGCCTCTGGGCTAATAGGACTACTTCTAATCTGTAAGAGCAGATCCCTGGACAGGCGAGGAATACAGGTATTTTGTCCTTGAAGTAACCTTTCAGAAATTCTGAGAATTT
PRIMER_LEFT_EXPLAIN=considered 2597, overlap target 32, GC content failed 195, low tm 1010, high tm 658, high end compl 23, high repeat similarity 8, ok 671
PRIMER_RIGHT_EXPLAIN=considered 2435, overlap target 50, low tm 1068, high tm 632, ok 685
PRIMER_PAIR_EXPLAIN=considered 18, high end compl 16, ok 2
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=288.237505
PRIMER_LEFT_0_PENALTY=263.479042
PRIMER_RIGHT_0_PENALTY=24.758462
PRIMER_LEFT_0_SEQUENCE=TTGCAAATGAAAACAATTTTGAA
PRIMER_RIGHT_0_SEQUENCE=TGAAAGGTTACTTCAAGGACAAAA
PRIMER_LEFT_0=23,23
PRIMER_RIGHT_0=302,24
PRIMER_LEFT_0_TM=59.521
PRIMER_RIGHT_0_TM=59.242
PRIMER_LEFT_0_GC_PERCENT=21.739
PRIMER_RIGHT_0_GC_PERCENT=33.333
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=260.000000
PRIMER_RIGHT_0_POSITION_PENALTY=20.000000
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=280
PRIMER_PAIR_0_PRODUCT_TM=74.6
=
SEQUENCE_ID=F7_X1
SEQUENCE_TARGET=298,68
SEQUENCE_TEMPLATE=ATTTGAAGTGTTGGTGCCACACACACCTTAACACCTGCACGCTGGCAACAAAACCGTCCGCTCTGCAGCACAGCTGGGGTCACCTGACCTTTCTCCTGTCCCCCCCACTTGAGCTCAGTGGCTGGGCAGCAGGGGATGCATGGCCACTGGCGGCCAGGTGCAGCTCTCAGCTGGGGTGTTCAGAGGACGCCTGTGTCCTCCCCTCCCCCATCCCTCTGTCACCCTTGGAGGCAGAGAACTTTGCCCGTCAGTCCCATGGGGAATGTCAACAGGCAGGGGCAGCACTGCAGAGATTTCATCATGGTCTCCCAGGCCCTCAGGCTCCTCTGCCTTCTGCTTGGGCTTCAGGGCTGCCTGGCTGCAGGTGCGTCCGGGGAGGTTTTCTCCATAAACTTGGTGGAAGGGCAGTGGGCAAATCCAGGAGCCAGCCCGGGCTTCCCAAACCCCGCCCTTGCTCCGGACACCCCCATCCACCAGGAGGGTTTTCTGGCGGCTCCTGTTCAATTTCTTTCCTTCTAGAAACCAGCATCCAGGCACAGGAGGGGAGGCCCTTCTTGGTAGCCCAGGCTTTGGCGGGATTATTTTTCAAAGAACTTTAGGAGTGGGTGGTGCTTTCCTGGCCCCCATGGCCCTGCCTGTGAGGTCGGACAAGCGCAGGGAGTCT
PRIMER_LEFT_EXPLAIN=considered 3572, overlap target 289, low tm 118, high tm 2723, long poly-x seq 23, ok 419
PRIMER_RIGHT_EXPLAIN=considered 3734, overlap target 289, GC content failed 17, low tm 289, high tm 2735, high end compl 1, ok 403
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=42.860415
PRIMER_LEFT_0_PENALTY=0.295503
PRIMER_RIGHT_0_PENALTY=42.564912
PRIMER_LEFT_0_SEQUENCE=GCAGCACTGCAGAGATTTCA
PRIMER_RIGHT_0_SEQUENCE=GAGAAAACCTCCCCGGAC
PRIMER_LEFT_0=278,20
PRIMER_RIGHT_0=385,18
PRIMER_LEFT_0_TM=60.296
PRIMER_RIGHT_0_TM=59.435
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=15.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=40.000000
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=9.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=108
PRIMER_PAIR_0_PRODUCT_TM=79.8
=
SEQUENCE_ID=F7_X2
SEQUENCE_TARGET=298,70
SEQUENCE_TEMPLATE=TGTGTGGGGTGTGGGGATAGGGTCAGGGGATGGCGTGGGGGGTGTGGCATGGGGATGGCACGTGTGGCATGGGGATGGGGATGGGGGGTGGGGCATGGCCGAGTGGGGCTGGGGCTGGGAATGGTGAGTGGGGCATGGGGATGGCGAGTAGGGGGTGTGGCGTGAGGATGGCTAGTGGGGCGTGGGGATGGCGTGTGGGGATGGCGAGTGGGGGGTGGGCTGTGAGGGACAGTGCCTGGGATGTGGCTGCAGCCCTAGCTCACAGCATGGCCTTATGACCCCGGCCACCTTCCTGCCCAGGCGGGGTCGCTAAGGCCTCAGGAGGAGAAACACGGGACATGCCGTGGAAGCCGGGGCCTCACAGAGGTGAGCAGGGACTGCCACTGGTTTTGTCCTGGGGCCCAGTGGGGGCAACATCACCTCCTTCCCCTCCCATGGCAAAGAGCCAGCCCGCGGGGTGGCTACTGCAGTGCCCCCCAAGGAGGGTGTTCCCTGCTCGAGAGGAAGTGACCGCTCCAGCTTGGCCTTCCCTGGGACTGGGGTGCAGGCGATTTTATCTTCTTTGCTCCATTCTGTTCCTTCCAGATAATCGTGTGTTCTTCATCAGGTTTTCCTCAGTTCTTGAGAGCTTTTCTGATGCAAATCTGCTTTCACCCCAGGGCGGTC
PRIMER_LEFT_EXPLAIN=considered 3301, overlap target 289, GC content failed 28, low tm 14, high tm 2710, high end compl 1, long poly-x seq 65, ok 194
PRIMER_RIGHT_EXPLAIN=considered 3601, overlap target 289, GC content failed 16, low tm 390, high tm 2278, long poly-x seq 22, ok 606
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=306.817739
PRIMER_LEFT_0_PENALTY=304.241567
PRIMER_RIGHT_0_PENALTY=2.576172
PRIMER_LEFT_0_SEQUENCE=CATGGCCTTATGACCCCG
PRIMER_RIGHT_0_SEQUENCE=CAGTGGCAGTCCCTGCTC
PRIMER_LEFT_0=265,18
PRIMER_RIGHT_0=385,18
PRIMER_LEFT_0_TM=62.242
PRIMER_RIGHT_0_TM=60.576
PRIMER_LEFT_0_GC_PERCENT=61.111
PRIMER_RIGHT_0_GC_PERCENT=66.667
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_PAIR_0_LIBRARY_MISPRIMING=15.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=300.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=12.9000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=121
PRIMER_PAIR_0_PRODUCT_TM=82.1
=
SEQUENCE_ID=F7_X2'
SEQUENCE_TARGET=298,165
SEQUENCE_TEMPLATE=AGCCTCACGTTTACCGCGGCGCCCGCAGCCCCCTTCGCCCGCTTCCGCGCGTGCCCCCGAGCGCGCCCTCGGGATCAGCCCCCGGAAGCAGAGAGGCCAGGCCGGGAAGGATGGGCGAACGGGGTGGCTGACCCGGGAGCACGGCAGGGAGGACACCCAGCCAGGCCCGCGAGCAGCGCCGCTCCCCTCCTCCAGGACGGGCGGGAACCTGCGATGCCCCCGCCGCGTGGGCCGTGGGGCGGTCTCCGAGGCACTGGGCGGGGCACGCGGTGGGCGCTTCACGGAACTCGCATTTCCCAGTCTTCGTAACCCAGGAGGAAGCCCACGGCGTCCTGCACCGGCGCCGGCGCGCCAACGCGTTCCTGGAGGAGCTGCGGCCGGGCTCCCTGGAGAGGGAGTGCAAGGAGGAGCAGTGCTCCTTCGAGGAGGCCCGGGAGATCTTCAAGGACGCGGAGAGGACGGTGAGCCCAGCCTCGGGGCGCCCCGCGCGGACACTGCACGGCGGCGGTGAACCAGGCCGCGTGGGGCCGCCTGCGTCTCTTTGGCTGCGGCCTGTGGGCGGCGAACACGCAGCGGCGCCCGCGCGCGCGCTCTCTCTGCGGGGGTCGCTTTCCGCCCGGGGTGACTCCGCTTTCCTGGGCGATGCCCCCACCCCCAGGCACGCGCTCTCCCCGTGCGGCCGCACCGCGCATGCCGGTTTTCACATCAGAAAATACGATTTGCACAAGCACACTTAGGGTGTCCCCCTTAACTTCCCAAGG
PRIMER_LEFT_EXPLAIN=considered 4704, overlap target 289, GC content failed 1097, low tm 35, high tm 3097, high end compl 6, ok 180
PRIMER_RIGHT_EXPLAIN=considered 4704, overlap target 289, GC content failed 1078, low tm 138, high tm 2874, ok 325
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=1385.802240
PRIMER_LEFT_0_PENALTY=62.138031
PRIMER_RIGHT_0_PENALTY=1323.664208
PRIMER_LEFT_0_SEQUENCE=CTTCACGGAACTCGCATTT
PRIMER_RIGHT_0_SEQUENCE=AGCCAAAGAGACGCAGGC
PRIMER_LEFT_0=276,19
PRIMER_RIGHT_0=546,18
PRIMER_LEFT_0_TM=58.862
PRIMER_RIGHT_0_TM=61.664
PRIMER_LEFT_0_GC_PERCENT=47.368
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=60.000000
PRIMER_RIGHT_0_POSITION_PENALTY=1320.000000
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=271
PRIMER_PAIR_0_PRODUCT_TM=86.4
=
SEQUENCE_ID=F7_X3
SEQUENCE_TARGET=298,29
SEQUENCE_TEMPLATE=CAGAGCCTGTGTACCTCGTCCCAGGTCCACAGCTCAGCGACAGAAGAGTCAGGGTTGAACCTCGGGTGTTCTGACTTGGGAGCAGGAAATGTGTGGTCACCCATAGTTCCAGATGTCCTGGGGAGGGGCCAAGATTAGAAGAAACCTACCTCAGCTCCAGAGGAAAGTCTGGCTTCCTGAGCCCACCCCGCCAGACCCAGGTCCAAGTCCCCCAACCCCAGTTCATGGTGTGTCCAGTGCTTACCGTTGGGTGCTCTGGTGAAGGTGCATCTCACGAGGCTTGCTCTCTTGTTCCTTCAGAAGCTGTTCTGGATTTCTTACAGTGGTGAGTGGATGATCACCACCAGTCCTGCCTGCAACCCTTCTCAGCTTACTGACACCAGCCCACTCCACAGATGGGGACCAGTGTGCCTCAAGTCCATGCCAGAATGGGGGCTCCTGCAAGGACCAGCTCCAGTCCTATATCTGCTTCTGCCTCCCTGCCTTCGAGGGCCGGAACTGTGAGACGCGTAAGGCCCCACTTTGGGTCCCATATTTGCAGAGGGCCCTGGGGAGCTGGTGGAGGTGGCCTGGCCAACCGGGCTGCAGGGTGCAACAACCTGGTGGGGTGTGTAGGCCGGGCATT
PRIMER_LEFT_EXPLAIN=considered 3344, overlap target 289, GC content failed 2, low tm 424, high tm 1910, ok 719
PRIMER_RIGHT_EXPLAIN=considered 3344, overlap target 289, low tm 284, high tm 2312, high any compl 8, high end compl 13, high repeat similarity 7, ok 431
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=303.683583
PRIMER_LEFT_0_PENALTY=1.510814
PRIMER_RIGHT_0_PENALTY=302.172769
PRIMER_LEFT_0_SEQUENCE=GGCTTGCTCTCTTGTTCCTTC
PRIMER_RIGHT_0_SEQUENCE=GTTGCAGGCAGGACTGGT
PRIMER_LEFT_0=277,21
PRIMER_RIGHT_0=359,18
PRIMER_LEFT_0_TM=60.511
PRIMER_RIGHT_0_TM=59.827
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, MIR2 Repetitive element - a consensus
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=300.000000
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=83
PRIMER_PAIR_0_PRODUCT_TM=73.4
=
SEQUENCE_ID=F7_X4
SEQUENCE_TARGET=298,118
SEQUENCE_TEMPLATE=GTCACCCATAGTTCCAGATGTCCTGGGGAGGGGCCAAGATTAGAAGAAACCTACCTCAGCTCCAGAGGAAAGTCTGGCTTCCTGAGCCCACCCCGCCAGACCCAGGTCCAAGTCCCCCAACCCCAGTTCATGGTGTGTCCAGTGCTTACCGTTGGGTGCTCTGGTGAAGGTGCATCTCACGAGGCTTGCTCTCTTGTTCCTTCAGAAGCTGTTCTGGATTTCTTACAGTGGTGAGTGGATGATCACCACCAGTCCTGCCTGCAACCCTTCTCAGCTTACTGACACCAGCCCACTCCACAGATGGGGACCAGTGTGCCTCAAGTCCATGCCAGAATGGGGGCTCCTGCAAGGACCAGCTCCAGTCCTATATCTGCTTCTGCCTCCCTGCCTTCGAGGGCCGGAACTGTGAGACGCGTAAGGCCCCACTTTGGGTCCCATATTTGCAGAGGGCCCTGGGGAGCTGGTGGAGGTGGCCTGGCCAACCGGGCTGCAGGGTGCAACAACCTGGTGGGGTGTGTAGGCCGGGCATTCAGGGCTCAGCCCAGTTGGAAATTGGTCTAGGTGACCTTTAAATCCCTTCCAGTCTGAGGTCTTTGACAGGGACCCAAGGTTCTGATTATCAGACTCAGTGGCCCCCTTCGCGGTCCCGGCCCTGGGCAACTTCTCAGCCCTGGAGACTGGCCCAGTTGAGAGTCCCTGTGTCCCGTGTGCCCA
PRIMER_LEFT_EXPLAIN=considered 4234, overlap target 289, GC content failed 2, low tm 537, high tm 2588, ok 818
PRIMER_RIGHT_EXPLAIN=considered 4234, overlap target 289, GC content failed 81, low tm 378, high tm 2891, ok 595
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=6.323804
PRIMER_LEFT_0_PENALTY=2.096539
PRIMER_RIGHT_0_PENALTY=4.227266
PRIMER_LEFT_0_SEQUENCE=GACACCAGCCCACTCCAC
PRIMER_RIGHT_0_SEQUENCE=GACCCAAAGTGGGGCCTT
PRIMER_LEFT_0=280,18
PRIMER_RIGHT_0=433,18
PRIMER_LEFT_0_TM=60.097
PRIMER_RIGHT_0_TM=62.227
PRIMER_LEFT_0_GC_PERCENT=66.667
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=154
PRIMER_PAIR_0_PRODUCT_TM=81.0
=
SEQUENCE_ID=F7_X5
SEQUENCE_TARGET=298,145
SEQUENCE_TEMPLATE=GCTCGGAAGAGTGGTCACCCTGGAGGCCACTGCCCACCAGCCAGGCACCCCCCAAATGCAACCGCAGCCAGCACTGCCAGCCACTGGCAAGGCTGTTCAGACATGTGGCTCCTCTGATCCACGCCTTGTCCTTTGGATCAGTCCACGGAGCAGTGTGCCAAGCTCAGGCTCTGTCACCCACAGCTCATGCCACCTTCCAGGCAGAACACCACTGCTGACCCAGGGGCATGGCCACCCCGGGGGCTGGCGTCTCGCTGACCCCCAGAAGCCCCTCTCAGGGTGTCCCCTTCCTGTCCCCAGACAAGGATGACCAGCTGATCTGTGTGAACGAGAACGGCGGCTGTGAGCAGTACTGCAGTGACCACACGGGCACCAAGCGCTCCTGTCGGTGCCACGAGGGGTACTCTCTGCTGGCAGACGGGGTGTCCTGCACACCCACAGGTGACCAGGCTTCATGTCCCAGTCCCAGATGACACCAGTCCCTGTCCCACTAGGATTATCTTACTGGACAAAAGACGGGTGGGACTGGCCTTCACATCTACTGAGCACTAACTATGCACTGACCAATTGTGAGGTGGGATCTGGGCACCAAGGGTGGCACAGGCCAGCAGCGACCAGTGACTAGGATGGGCACCCTGGGGGCAATCCCTGAATGGCCTCAGGCCCCCTGCCAACTTCTAGGCAGACCAGGGGAGCCAAGCAAGGCACTATCTCACGTCCAACTGCCCACTCGCAGGAATC
PRIMER_LEFT_EXPLAIN=considered 4351, overlap target 289, GC content failed 139, low tm 152, high tm 3259, long poly-x seq 22, ok 490
PRIMER_RIGHT_EXPLAIN=considered 4504, overlap target 289, GC content failed 1, low tm 499, high tm 3030, high repeat similarity 2, ok 683
PRIMER_PAIR_EXPLAIN=considered 20, high any compl 8, high end compl 10, ok 2
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=84.356932
PRIMER_LEFT_0_PENALTY=62.311131
PRIMER_RIGHT_0_PENALTY=22.045801
PRIMER_LEFT_0_SEQUENCE=GGGTGTCCCCTTCCTGTC
PRIMER_RIGHT_0_SEQUENCE=TGGGACATGAAGCCTGGT
PRIMER_LEFT_0=277,18
PRIMER_RIGHT_0=461,18
PRIMER_LEFT_0_TM=60.311
PRIMER_RIGHT_0_TM=60.046
PRIMER_LEFT_0_GC_PERCENT=66.667
PRIMER_RIGHT_0_GC_PERCENT=55.556
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=60.000000
PRIMER_RIGHT_0_POSITION_PENALTY=20.000000
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=185
PRIMER_PAIR_0_PRODUCT_TM=82.1
=
SEQUENCE_ID=F7_X6
SEQUENCE_TARGET=298,114
SEQUENCE_TEMPLATE=GAAAAGTGAGCAGAGGGCGGTTTTAAGACGTAAGCCCTCTGTTTCCTCCAAAACCAGCCCTGACCATTGTCTCCTCAGCCAGCCACTTCTTCAAGGGCCTCTCATGGCCGGGCCCCACCAGTCAGGCCCAGCCGAGGCCCTGCCTTCCACCACCCCTGGGCCCTGGGAGCTCCTGCTCCTGGGGGCCTCCCATAGCCTCGGCCTCAAGGCCTCTCAGAGGATGGGTGTTTCTGAATCTTTCCTAGTGGCACGTTCATCCCTCACAAATCTCTGCATCTTTCTGACTTTTGTTTTACACAGTTGAATATCCATGTGGAAAAATACCTATTCTAGAAAAAAGAAATGCCAGCAAACCCCAAGGCCGAATTGTGGGGGGCAAGGTGTGCCCCAAAGGGGAGTGTCCATGGCAGGTAAGGCTTCCCCTGGCTTCAGGATTCCAAGCCCTGAGGGTCTTGAAGCCTTTTGAATGTGAACAACAGCTCTGGAAGGGAAAATGGGCAGGTCAGCCCAAGCCCACAGGCTCCAAGTCAGCACACCTAGCACCTCCAGCTCGCGGCACCCCCATGCTTTTAGTGGGGCAAGGAAGGAGAAAAGAAAACGACACTCACTGAGGGTCTACCCTGTGCAGAGAACCCTGCGAGATGCCCCATCCGAGTTGTCACGTCGTCCTCACGGTTACTCTTTGAGGTGGGATCTTTGCCTGATCTTTG
PRIMER_LEFT_EXPLAIN=considered 3891, overlap target 289, GC content failed 43, low tm 599, high tm 2265, long poly-x seq 44, ok 651
PRIMER_RIGHT_EXPLAIN=considered 3894, overlap target 289, GC content failed 11, low tm 463, high tm 2371, high end compl 1, long poly-x seq 44, ok 715
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=88.837406
PRIMER_LEFT_0_PENALTY=6.324793
PRIMER_RIGHT_0_PENALTY=82.512614
PRIMER_LEFT_0_SEQUENCE=GCATCTTTCTGACTTTTGTTTTACAC
PRIMER_RIGHT_0_SEQUENCE=ATCCTGAAGCCAGGGGAAG
PRIMER_LEFT_0=272,26
PRIMER_RIGHT_0=434,19
PRIMER_LEFT_0_TM=59.675
PRIMER_RIGHT_0_TM=61.513
PRIMER_LEFT_0_GC_PERCENT=34.615
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=80.000000
PRIMER_LEFT_0_END_STABILITY=5.4000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=163
PRIMER_PAIR_0_PRODUCT_TM=75.6
=
SEQUENCE_ID=F7_X7
SEQUENCE_TARGET=298,128
SEQUENCE_TEMPLATE=CTTTGCAAAATCAGGAGCATTGGATCAAAGCTATGTGAAGATCCTGTGAGGTGAACAGTGAAATCTCACAGCGACATTTGTATTCTTGGGCCGTGCCCAAGAGCACGTCTCGGCTAGAGAGGGGCACAGCCTCCCAGAGCCAGGTCTGAGCAGCTTTGCCTGGGAGGGATCTGCAAAGACCCCAGGATTTCAGAAAGAAATTGTGCAATGCCAGAGGTTCCTTGGCATGCCCGGGAGGGCGAGTCATCAGAGAAACAATGACAGCAATGTGACTTCCACACCTCCTGTCCCCCCGCCCAGGTCCTGTTGTTGGTGAATGGAGCTCAGTTGTGTGGGGGGACCCTGATCAACACCATCTGGGTGGTCTCCGCGGCCCACTGTTTCGACAAAATCAAGAACTGGAGGAACCTGATCGCGGTGCTGGGTGGGTACCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTTCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCGCTGTCCGACCGCGGTGCTGGGTGGGTACCACTCTCCCCTGTCTGACCGCAGCTCTCAAGTGTCTCAGGGGCTGTGGCTCTGGGCTTCGTGCTGTCACTTCCACAGACAGACAGACATCCCCAAAAGGGGAGCAAC
PRIMER_LEFT_EXPLAIN=considered 4032, overlap target 289, GC content failed 6, low tm 577, high tm 2278, long poly-x seq 44, ok 838
PRIMER_RIGHT_EXPLAIN=considered 4181, overlap target 289, GC content failed 10, low tm 236, high tm 3155, high end compl 5, long poly-x seq 22, ok 464
PRIMER_PAIR_EXPLAIN=considered 126, high any compl 28, high end compl 97, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=363.438040
PRIMER_LEFT_0_PENALTY=301.362873
PRIMER_RIGHT_0_PENALTY=62.075168
PRIMER_LEFT_0_SEQUENCE=AGCAATGTGACTTCCACACCT
PRIMER_RIGHT_0_SEQUENCE=CGGACAGGGGAGAGTGGTA
PRIMER_LEFT_0=262,21
PRIMER_RIGHT_0=447,19
PRIMER_LEFT_0_TM=59.637
PRIMER_RIGHT_0_TM=61.075
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=63.158
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=300.000000
PRIMER_RIGHT_0_POSITION_PENALTY=60.000000
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=186
PRIMER_PAIR_0_PRODUCT_TM=80.9
=
SEQUENCE_ID=F7_X8
SEQUENCE_TARGET=298,600
SEQUENCE_TEMPLATE=GCCGGGAAGAACTGTGGAGAATCGGGGCACGCCCTGTCCTCCCAGCTGCCAGGGCACAGCATCCCTTCCCCACCTGCAACACCCAGACCCCAGATTCACCCCAGTTCACTTGTCCCCACACGAGCCACAGGCTGCCACCTGGGGCAGGCTGGCCCACCTTGGGGTTAGATGCAGGTCCCCTTGCCCCAGAAGGAGACTGCAGCCCCTGCAGACCTAGAAATGGCCACAGCCCATCCCCATGCACCAGGGGGTGAGGTGGCAGGTGGTGGAAAGGGCCTGAGGGGGGCTTCTTCCTTCCAGGCGAGCACGACCTCAGCGAGCACGACGGGGATGAGCAGAGCCGGCGGGTGGCGCAGGTCATCATCCCCAGCACGTACGTCCCGGGCACCACCAACCACGACATCGCGCTGCTCCGCCTGCACCAGCCCGTGGTCCTCACTGACCATGTGGTGCCCCTCTGCCTGCCCGAACGGACGTTCTCTGAGAGGACGCTGGCCTTCGTGCGCTTCTCATTGGTCAGCGGCTGGGGCCAGCTGCTGGACCGTGGCGCCACGGCCCTGGAGCTCATGGTCCTCAACGTGCCCCGGCTGATGACCCAGGACTGCCTGCAGCAGTCACGGAAGGTGGGAGACTCCCCAAATATCACGGAGTACATGTTCTGTGCCGGCTACTCGGATGGCAGCAAGGACTCCTGCAAGGGGGACAGTGGAGGCCCACATGCCACCCACTACCGGGGCACGTGGTACCTGACGGGCATCGTCAGCTGGGGCCAGGGCTGCGCAACCGTGGGCCACTTTGGGGTGTACACCAGGGTCTCCCAGTACATCGAGTGGCTGCAAAAGCTCATGCGCTCAGAGCCACGCCCAGGAGTCCTCCTGCGAGCCCCATTTCCCTAGCCCAGCAGCCCTGGCCTGTGGAGAGAAAGCCAAGGCTGCGTCGAACTGTCCTGGCACCAAATCCCATATATTCTTCTGCAGTTAATGGGGTAGAGGAGGGCATGGGAGGGAGGGAGAGGTGGGGAGGGAGACAGAGACAGAAACAGAGAGAGACAGAGACAGAGAGAGACTGAGGGAGAGACTCTGAGGACATGGAGAGAGACTCAAAGAGACTCCAAGATTCAAAGAGACTAATAGAGACACAGAGATGGAATAGAAAAGATGAGAGGCAGAGGCAGACAGGCGCTGGACAGAGGGGCA
PRIMER_LEFT_EXPLAIN=considered 8901, overlap target 289, GC content failed 87, low tm 201, high tm 7523, long poly-x seq 22, ok 779
PRIMER_RIGHT_EXPLAIN=considered 9054, overlap target 289, GC content failed 79, low tm 1105, high tm 6374, high end compl 1, ok 1206
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=447.046454
PRIMER_LEFT_0_PENALTY=304.949476
PRIMER_RIGHT_0_PENALTY=142.096979
PRIMER_LEFT_0_SEQUENCE=GTGGAAAGGGCCTGAGGG
PRIMER_RIGHT_0_SEQUENCE=TTCTCTCCACAGGCCAGG
PRIMER_LEFT_0=265,18
PRIMER_RIGHT_0=922,18
PRIMER_LEFT_0_TM=62.949
PRIMER_RIGHT_0_TM=59.903
PRIMER_LEFT_0_GC_PERCENT=66.667
PRIMER_RIGHT_0_GC_PERCENT=61.111
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, reverse MIR2 Repetitive element - a consensus
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=300.000000
PRIMER_RIGHT_0_POSITION_PENALTY=140.000000
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=658
PRIMER_PAIR_0_PRODUCT_TM=85.8
=
SEQUENCE_ID=HCF2_X2
SEQUENCE_TARGET=298,909
SEQUENCE_TEMPLATE=CTCCTCCCTGGGATGCCTGAGCTCCCAGGATTCTAGAAGGTTAGTTTTGCAAACCTTTAAAGAAGGGATTTTCATCAAGGGGCCCACAGATCCTTCATTGAGGTTTATGAGTCCCACATCAAAGGTTGGGTGTCTATCTACATCAGATTCTCTTAAAGTCCATGATCCTAAAACAGTTAAGAACTAATGCTGTGAGGGCCTCTTCCTGGGTCAAAGCCACAGGGAACCTGCCATGTGGATGCTGCAGCGGGGTGTGGATCAGCCAGGCCGCCTTTCACTGTGTTCTGTTTTCCCTCCCAGCTTTAGCTCCGCCAAAATGAAACACTCATTAAACGCACTTCTCATTTTCCTCATCATAACATCTGCGTGGGGTGGGAGCAAAGGCCCGCTGGATCAGCTAGAGAAAGGAGGGGAAACTGCTCAGTCTGCAGATCCCCAGTGGGAGCAGTTAAATAACAAAAACCTGAGCATGCCTCTTCTCCCTGCCGACTTCCACAAGGAAAACACCGTCACCAACGACTGGATTCCAGAGGGGGAGGAGGACGACGACTATCTGGACCTGGAGAAGATATTCAGTGAAGACGACGACTACATCGACATCGTCGACAGTCTGTCAGTTTCCCCGACAGACTCTGATGTGAGTGCTGGGAACATCCTCCAGCTTTTTCATGGCAAGAGCCGGATCCAGCGTCTTAACATCCTCAACGCCAAGTTCGCTTTCAACCTCTACCGAGTGCTGAAAGACCAGGTCAACACTTTCGATAACATCTTCATAGCACCCGTTGGCATTTCTACTGCGATGGGTATGATTTCCTTAGGTCTGAAGGGAGAGACCCATGAACAAGTGCACTCGATTTTGCATTTTAAAGACTTTGTTAATGCCAGCAGCAAGTATGAAATCACGACCATTCATAATCTCTTCCGTAAGCTGACTCATCGCCTCTTCAGGAGGAATTTTGGGTACACACTGCGGTCAGTCAATGACCTTTATATCCAGAAGCAGTTTCCAATCCTGCTTGACTTCAAAACTAAAGTAAGAGAGTATTACTTTGCTGAGGCCCAGATAGCTGACTTCTCAGACCCTGCCTTCATATCAAAAACCAACAACCACATCATGAAGCTCACCAAGGGCCTCATAAAAGATGCTCTGGAGAATATAGACCCTGCTACCCAGATGATGATTCTCAACTGCATCTACTTCAAAGGTAAGAGGCACCTTTACAGTTCTCACAGCAAACCCACAACATACTATTTTTGTATGTGGGTAGATTGAATGCCAAGAACTGTACTGTAGCTATAATTTATCCAGGAAAACTAGACACAAGATTGACTCTGGAACGGGGACAGGGAAGGCCAAGCTGAAGTGACAGTAGCATCTGACACTTACTGAGCCCTAACTCTGTGCTTTAACACAGCCTTGTGAGGTCATCACTGTTATTAGCATCCCCATTTTACAGAGGAAGCCACCAACACATGAAGTAAAAGGATGGGCTGGGCGCGGTGGC
PRIMER_LEFT_EXPLAIN=considered 12144, overlap target 289, GC content failed 12, low tm 3242, high tm 5342, ok 3259
PRIMER_RIGHT_EXPLAIN=considered 12144, overlap target 289, GC content failed 15, low tm 3651, high tm 4687, high end compl 7, ok 3495
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=43.762258
PRIMER_LEFT_0_PENALTY=0.056936
PRIMER_RIGHT_0_PENALTY=43.705322
PRIMER_LEFT_0_SEQUENCE=TGTGTTCTGTTTTCCCTCCC
PRIMER_RIGHT_0_SEQUENCE=TGTGAGAACTGTAAAGGTGCCTC
PRIMER_LEFT_0=278,20
PRIMER_RIGHT_0=1231,23
PRIMER_LEFT_0_TM=59.943
PRIMER_RIGHT_0_TM=60.705
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=47.826
PRIMER_LEFT_0_SELF_ANY=1.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=40.000000
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=954
PRIMER_PAIR_0_PRODUCT_TM=78.6
=
SEQUENCE_ID=HCF2_X3
SEQUENCE_TARGET=298,278
SEQUENCE_TEMPLATE=TTTGACAGATGTGACAGATGAAGAAATAGAAGCGAGTTAGGTGCCTTACCATGGTCAAACAACTAGTTCGTATCAGACCCTACTCCAGAAACTATTCCAGTCCGGGTAACCTCTCGTTAACCTCTCTTGTTAGAAATGCAAATTTCTGCCCAAATCAGGCCTCAGGAATCAAGAGACTGTGGGGTCGGCTCTGCAGGCTATCTGAATGAGGCCTCCAGGGAAATCAGATTCACTCTCAAGGGTGAGACGATTTCCCTAAAGGAACCTTCTCATAACAGCCTCTTCCTGTGGCCTTTACAGGATCCTGGGTGAATAAATTCCCAGTGGAAATGACACACAACCACAACTTCCGGCTGAATGAGAGAGAGGTAGTTAAGGTTTCCATGATGCAGACCAAGGGGAACTTCCTCGCAGCAAATGACCAGGAGCTGGACTGCGACATCCTCCAGCTGGAATACGTGGGGGGCATCAGCATGCTAATTGTGGTCCCACACAAGATGTCTGGGATGAAGACCCTCGAAGCGCAACTGACACCCCGGGTGGTGGAGAGATGGCAAAAAAGCATGACAAACAGGTATTTCACACTGTGTGTTTGTTCTTTTGAGCTCCCAGATGCTGGGGGTGTCTGGGAATACTGGAAAATGGATCATTTTTTTAAAAAGGGAGAATTATGTACAAGTACCCAAGAACTTCCATACAGGGCCACTCTGTTAATTCAGCCCCAATTTGTTGCTTGAGATAAGAGATGATTAGAGAGCATTCATAAGGGACACATCTGCCCTCTAGGGGCCAGTTTCAGAAGTTAGAGGCAGATGACTTAGAGACAGCTTGGTGCTTGCTTTGTGGCTTCGAGTCCCAGCTTCATCATCCCTAA
PRIMER_LEFT_EXPLAIN=considered 5543, overlap target 289, low tm 1266, high tm 2461, long poly-x seq 38, ok 1489
PRIMER_RIGHT_EXPLAIN=considered 5393, overlap target 289, GC content failed 28, low tm 1331, high tm 2335, high any compl 19, high end compl 7, long poly-x seq 67, ok 1317
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=145.727655
PRIMER_LEFT_0_PENALTY=1.210543
PRIMER_RIGHT_0_PENALTY=144.517111
PRIMER_LEFT_0_SEQUENCE=CCTCTTCCTGTGGCCTTTAC
PRIMER_RIGHT_0_SEQUENCE=GCTCAAAAGAACAAACACACAGT
PRIMER_LEFT_0=278,20
PRIMER_RIGHT_0=605,23
PRIMER_LEFT_0_TM=58.789
PRIMER_RIGHT_0_TM=58.483
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=39.130
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=140.000000
PRIMER_LEFT_0_END_STABILITY=6.0000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=328
PRIMER_PAIR_0_PRODUCT_TM=78.8
=
SEQUENCE_ID=HCF2_X4
SEQUENCE_TARGET=298,149
SEQUENCE_TEMPLATE=ATGCCTTTTTAAACCATAAACCAGGAAAAGTTCTGCTGCTAACCTTGAAGATAGGAAACGAACCATACAGTCTCAAGGAAATAATCATGCAACAGAAAACACACCTCAGTTTTCAGTAGCGGAATTACAAAGGAGTGTGCTTCCTAAAATCCTCAACTGACAGTCCCGGAATATAAATTTTAATAAGTGCTATATCAATTCTGTGATAAATATAACCCGTGGCCCTTTAAAGGGAAAATCATGATTCTTTTGTAACTTGTGGTTCAATAAAACTGGGCCCCCCTTTCCTTTTCTGTCTAGAACTCGAGAAGTGCTTCTGCCGAAATTCAAGCTGGAGAAGAACTACAATCTAGTGGAGTCCCTGAAGTTGATGGGGATCAGGATGCTGTTTGACAAAAATGGCAACATGGCAGGCATCTCAGACCAAAGGATCGCCATCGACCTGGTAACCACTCCCTTGTCCACCCCCGACCCGTCCCCAGGGTCTGCCTCAGCACAGCCCCACCTCCACTTGCCCTTCCTACCCACCCCCCAATCTCATGTCCCAGCTTGGGGTGCTGAGTCTGCTCTTCGGCCTGGGTGGGATACACAGAATGCCTAGTTTCATGGATGCCAGCTGGAGAGCACGGCACCTGGCAGACACTTACTGGGCAGGGGGGATCCCAAGAGCAGCCATGGGGTGAGCCCCACTCCCGCTGACACCAGAGACAGGGGAGACATGTGCTGCGGTCTGGGAAATAGCTAC
PRIMER_LEFT_EXPLAIN=considered 4391, overlap target 289, GC content failed 128, low tm 1570, high tm 1096, long poly-x seq 22, ok 1286
PRIMER_RIGHT_EXPLAIN=considered 4238, overlap target 289, GC content failed 20, low tm 420, high tm 2770, long poly-x seq 44, ok 695
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=1.650642
PRIMER_LEFT_0_PENALTY=0.451656
PRIMER_RIGHT_0_PENALTY=1.198986
PRIMER_LEFT_0_SEQUENCE=CCCCCTTTCCTTTTCTGTCT
PRIMER_RIGHT_0_SEQUENCE=GGTGGACAAGGGAGTGGTT
PRIMER_LEFT_0=278,20
PRIMER_RIGHT_0=465,19
PRIMER_LEFT_0_TM=59.548
PRIMER_RIGHT_0_TM=59.801
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=1.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, MIR2 Repetitive element - a consensus
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=0.000000
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=188
PRIMER_PAIR_0_PRODUCT_TM=77.0
=
SEQUENCE_ID=HCF2_X5
SEQUENCE_TARGET=298,850
SEQUENCE_TEMPLATE=CCCGGAGAAGTGCGCAGCAGTGTGGGGAGCTGGAGCTGGGGTGGCTGTCCTGCACCAGCCCCCACGACCCTCAGACCACAGGCACTGCCAAGAGGGAACATGAACCTAGCCGGCCTCTAAGTGCAACGGCTGCCCCTGACAGGTGGTGACAGATATTTTCAAGAGTGACTCTGACCAGCTGTGATTTCCACCTTACATGTTGTCTTTGGATCCTTTCCCTGAATGATATGAGATTGTGCTGGGAACTCTAGCCCTCTGTGTGCTGACCTCCAGAATCTGACAACTTTCCTTTCCAAACAGTTCAAGCACCAAGGCACGATCACAGTGAACGAGGAAGGCACCCAAGCCACCACTGTGACCACGGTGGGGTTCATGCCGCTGTCCACCCAAGTCCGCTTCACTGTCGACCGCCCCTTTCTTTTCCTCATCTACGAGCATCGCACCAGCTGCCTGCTCTTCATGGGAAGAGTGGCCAACCCCAGCAGGTCCTAGAGGTGGAGGTCTAGGTGTCTGAAGTGCCTTGGGGGCACCCTCATTTTGTTTCCATTCCAACAACGAGAACAGAGATGTTCTGGCATCATTTACGTAGTTTACGCTACCAATCTGAATTCGAGGCCCATATGAGAGGAGCTTAGAAACGACCAAGAAGAGAGGCTTGTTGGAATCAATTCTGCACAATAGCCCATGCTGTAAGCTCATAGAAGTCACTGTAACTGTAGTGTGTCTGCTGTTACCTAGAGGGTCTCACCTCCCCACTCTTCACAGCAAACCTGAGCAGCGCGTCCTAAGCACCTCCCGCTCCGGTGACCCCATCCTTGCACACCTGACTCTGTCACTCAAGCCTTTCTCCACCAGGCCCCTCATCTGAATACCAAGCACAGAAATGAGTGGTGTGACTAATTCCTTACCTCTCCCAAGGAGGGTACACAACTAGCACCATTCTTGATGTCCAGGGAAGAAGCCACCTCAAGACATATGAGGGGTGCCCTGGGCTAATGTTAGGGCTTAATTTTCTCAAAGCCTGACCTTTCAAATCCATGATGAATGCCATCAGTCCCTCCTGCTGTTGCCTCCCTGTGACCTGGAGGACAGTGTGTGCCATGTCTCCCATACTAGAGATAAATAAATGTAGCCACATTTACTGTGTATCTGTTATAATTCTCTATTTTTTGAAGCTCAAATATCAAAAGCCAAATCCAAATTCCTGGATAACTCCAGGTATGATAAAGGCTGAGAGGAAGTCACTTGAGCACCACAATGTGCCACAGCAGGGCATGTTCTCAGGACAGGACAGGTGTGTGCTGAATCCTGGGGAGGGTCTGTGCAGTACCCCAGAACTGTGGGGTGCTAAGTGGCACACAAGCCCCAGGGCTCCCACAGTCTATGCCAGGCTGCTGCAGCTTTCATCCCTCATACCTGGTCCTGCAGTGGGTCTGGTTTGACAGA
PRIMER_LEFT_EXPLAIN=considered 11554, overlap target 289, GC content failed 7, low tm 2490, high tm 6121, ok 2647
PRIMER_RIGHT_EXPLAIN=considered 11432, overlap target 289, GC content failed 57, low tm 2518, high tm 5947, high end compl 12, long poly-x seq 20, ok 2589
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=466.578446
PRIMER_LEFT_0_PENALTY=3.339047
PRIMER_RIGHT_0_PENALTY=463.239398
PRIMER_LEFT_0_SEQUENCE=TCTGACAACTTTCCTTTCCAAAC
PRIMER_RIGHT_0_SEQUENCE=TTGGCTTTTGATATTTGAGCTTC
PRIMER_LEFT_0=275,23
PRIMER_RIGHT_0=1193,23
PRIMER_LEFT_0_TM=59.661
PRIMER_RIGHT_0_TM=59.761
PRIMER_LEFT_0_GC_PERCENT=39.130
PRIMER_RIGHT_0_GC_PERCENT=34.783
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=0.000000
PRIMER_RIGHT_0_POSITION_PENALTY=460.000000
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=919
PRIMER_PAIR_0_PRODUCT_TM=79.6
=
SEQUENCE_ID=HMGCR_X1
SEQUENCE_TARGET=74,164
SEQUENCE_TEMPLATE=CCAGGCTGAGCGTCGGCGCCGGGGTTCGGTGGCCTCTAGTGAGATCTGGAGGATCCAAGGATTCTGTAGCTACAATGTTGTCAAGACTTTTTCGAATGCATGGCCTCTTTGTGGCCTCCCATCCCTGGGAAGTCATAGTGGGGACAGTGACACTGACCATCTGCATGATGTCCATGAACATGTTTACTGGTAACAATAAGATCTGTGGTTGGAATTATGAATGTCCAAAGTTTGAAGA
PRIMER_LEFT_EXPLAIN=considered 2030, GC content failed 67, low tm 611, high tm 842, high any compl 3, high end compl 38, ok 469
PRIMER_RIGHT_EXPLAIN=considered 1490, overlap target 65, low tm 478, high tm 584, ok 363
PRIMER_PAIR_EXPLAIN=considered 11, high end compl 10, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=24308.407718
PRIMER_LEFT_0_PENALTY=302.282278
PRIMER_RIGHT_0_PENALTY=24006.125439
PRIMER_LEFT_0_SEQUENCE=TGAGATCTGGAGGATCCAAG
PRIMER_RIGHT_0_SEQUENCE=TCTTCAAACTTTGGACATTCATAA
PRIMER_LEFT_0=39,20
PRIMER_RIGHT_0=237,24
PRIMER_LEFT_0_TM=57.718
PRIMER_RIGHT_0_TM=57.875
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=29.167
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=300.000000
PRIMER_RIGHT_0_POSITION_PENALTY=24000.000000
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=6.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=199
PRIMER_PAIR_0_PRODUCT_TM=74.8
=
SEQUENCE_ID=HMGCR_X2
SEQUENCE_TARGET=0,112
SEQUENCE_TEMPLATE=GGATGTTTTGAGCAGTGACATTATAATTCTGACAATAACACGATGCATAGCCATCCTGTATATTTACTTCCAGTTCCAGAATTTACGTCAACTTGGATCAAAATATATTTTG
PRIMER_LEFT_EXPLAIN=considered 770, GC content failed 5, low tm 460, high tm 81, ok 224
PRIMER_RIGHT_EXPLAIN=considered 770, GC content failed 11, low tm 448, high tm 81, high any compl 7, high end compl 1, ok 222
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=49011.420343
PRIMER_LEFT_0_PENALTY=20002.797253
PRIMER_RIGHT_0_PENALTY=29008.623090
PRIMER_LEFT_0_SEQUENCE=GGATGTTTTGAGCAGTGACA
PRIMER_RIGHT_0_SEQUENCE=AATATATTTTGATCCAAGTTGACGTA
PRIMER_LEFT_0=0,20
PRIMER_RIGHT_0=108,26
PRIMER_LEFT_0_TM=57.203
PRIMER_RIGHT_0_TM=57.377
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=26.923
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=20000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=29000.000000
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=109
PRIMER_PAIR_0_PRODUCT_TM=68.3
=
SEQUENCE_ID=HMGCR_X3
SEQUENCE_TARGET=0,88
SEQUENCE_TEMPLATE=GGTATTGCTGGCCTTTTCACAATTTTCTCAAGTTTTGTATTCAGTACAGTTGTCATTCACTTCTTAGACAAAGAATTGACAGGCTTGA
PRIMER_LEFT_EXPLAIN=considered 530, GC content failed 1, low tm 361, high tm 44, ok 124
PRIMER_RIGHT_EXPLAIN=considered 530, GC content failed 1, low tm 416, high tm 4, ok 109
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=40005.823174
PRIMER_LEFT_0_PENALTY=19002.290742
PRIMER_RIGHT_0_PENALTY=21003.532432
PRIMER_LEFT_0_SEQUENCE=GGTATTGCTGGCCTTTTCA
PRIMER_RIGHT_0_SEQUENCE=TCAAGCCTGTCAATTCTTTGT
PRIMER_LEFT_0=0,19
PRIMER_RIGHT_0=87,21
PRIMER_LEFT_0_TM=58.709
PRIMER_RIGHT_0_TM=57.468
PRIMER_LEFT_0_GC_PERCENT=47.368
PRIMER_RIGHT_0_GC_PERCENT=38.095
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=19000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=21000.000000
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=88
PRIMER_PAIR_0_PRODUCT_TM=68.0
=
SEQUENCE_ID=HMGCR_X4
SEQUENCE_TARGET=0,85
SEQUENCE_TEMPLATE=ATGAAGCTTTGCCCTTTTTCCTACTTTTGATTGACCTTTCCAGAGCAAGCACATTAGCAAAGTTTGCCCTCAGTTCCAACTCACA
PRIMER_LEFT_EXPLAIN=considered 500, low tm 151, high tm 175, ok 174
PRIMER_RIGHT_EXPLAIN=considered 500, low tm 123, high tm 202, ok 175
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=39005.482012
PRIMER_LEFT_0_PENALTY=20001.557050
PRIMER_RIGHT_0_PENALTY=19003.924962
PRIMER_LEFT_0_SEQUENCE=ATGAAGCTTTGCCCTTTTTC
PRIMER_RIGHT_0_SEQUENCE=TGTGAGTTGGAACTGAGGG
PRIMER_LEFT_0=0,20
PRIMER_RIGHT_0=84,19
PRIMER_LEFT_0_TM=58.443
PRIMER_RIGHT_0_TM=57.075
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=52.632
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=20000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=19000.000000
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=85
PRIMER_PAIR_0_PRODUCT_TM=70.2
=
SEQUENCE_ID=HMGCR_X5
SEQUENCE_TARGET=0,106
SEQUENCE_TEMPLATE=GGATGAAGTAAGGGAAAATATTGCTCGTGGAATGGCAATTTTAGGTCCTACGTTTACCCTCGATGCTCTTGTTGAATGTCTTGTGATTGGAGTTGGTACCATGTCA
PRIMER_LEFT_EXPLAIN=considered 710, low tm 212, high tm 246, high end compl 1, ok 251
PRIMER_RIGHT_EXPLAIN=considered 710, low tm 202, high tm 248, high end compl 1, high repeat similarity 1, ok 258
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=47007.389548
PRIMER_LEFT_0_PENALTY=25005.106680
PRIMER_RIGHT_0_PENALTY=22002.282868
PRIMER_LEFT_0_SEQUENCE=GGATGAAGTAAGGGAAAATATTGCT
PRIMER_RIGHT_0_SEQUENCE=TGACATGGTACCAACTCCAATC
PRIMER_LEFT_0=0,25
PRIMER_RIGHT_0=105,22
PRIMER_LEFT_0_TM=60.107
PRIMER_RIGHT_0_TM=59.717
PRIMER_LEFT_0_GC_PERCENT=36.000
PRIMER_RIGHT_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=25000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=22000.000000
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=106
PRIMER_PAIR_0_PRODUCT_TM=71.6
=
SEQUENCE_ID=HMGCR_X6
SEQUENCE_TARGET=0,107
SEQUENCE_TEMPLATE=GGGGTACGTCAGCTTGAAATTATGTGCTGCTTTGGCTGCATGTCAGTTCTTGCCAACTACTTCGTGTTCATGACTTTCTTCCCAGCTTGTGTGTCCTTGGTATTAGA
PRIMER_LEFT_EXPLAIN=considered 720, low tm 206, high tm 309, ok 205
PRIMER_RIGHT_EXPLAIN=considered 720, low tm 181, high tm 338, ok 201
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=41009.536490
PRIMER_LEFT_0_PENALTY=18004.862764
PRIMER_RIGHT_0_PENALTY=23004.673725
PRIMER_LEFT_0_SEQUENCE=GGGGTACGTCAGCTTGAA
PRIMER_RIGHT_0_SEQUENCE=TCTAATACCAAGGACACACAAGC
PRIMER_LEFT_0=0,18
PRIMER_RIGHT_0=106,23
PRIMER_LEFT_0_TM=57.137
PRIMER_RIGHT_0_TM=58.326
PRIMER_LEFT_0_GC_PERCENT=55.556
PRIMER_RIGHT_0_GC_PERCENT=43.478
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=18000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=23000.000000
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=107
PRIMER_PAIR_0_PRODUCT_TM=73.1
=
SEQUENCE_ID=HMGCR_X7
SEQUENCE_TARGET=0,117
SEQUENCE_TEMPLATE=GCTTTCTCGGGAAAGCCGCGAGGGTCGTCCAATTTGGCAGCTCAGCCATTTTGCCCGAGTTTTAGAAGAAGAAGAAAATAAGCCGAATCCTGTAACTCAGAGGGTCAAGATGATTAT
PRIMER_LEFT_EXPLAIN=considered 820, GC content failed 3, low tm 162, high tm 464, ok 191
PRIMER_RIGHT_EXPLAIN=considered 820, GC content failed 3, low tm 237, high tm 349, ok 231
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=59009.628979
PRIMER_LEFT_0_PENALTY=35003.015981
PRIMER_RIGHT_0_PENALTY=24006.612999
PRIMER_LEFT_0_SEQUENCE=GCGAGGGTCGTCCAATTT
PRIMER_RIGHT_0_SEQUENCE=ATAATCATCTTGACCCTCTGAGTT
PRIMER_LEFT_0=17,18
PRIMER_RIGHT_0=116,24
PRIMER_LEFT_0_TM=61.016
PRIMER_RIGHT_0_TM=57.387
PRIMER_LEFT_0_GC_PERCENT=55.556
PRIMER_RIGHT_0_GC_PERCENT=37.500
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=35000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=24000.000000
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=100
PRIMER_PAIR_0_PRODUCT_TM=72.4
=
SEQUENCE_ID=HMGCR_X8
SEQUENCE_TARGET=0,161
SEQUENCE_TEMPLATE=GTCTCTAGGCTTGGTTCTTGTTCATGCTCACAGTCGCTGGATAGCTGATCCTTCTCCTCAAAACAGTACAGCAGATACTTCTAAGGTTTCATTAGGACTGGATGAAAATGTGTCCAAGAGAATTGAACCAAGTGTTTCCCTCTGGCAGTTTTATCTCTCTA
PRIMER_LEFT_EXPLAIN=considered 1260, low tm 540, high tm 321, ok 399
PRIMER_RIGHT_EXPLAIN=considered 1260, low tm 530, high tm 315, high repeat similarity 20, ok 395
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=51007.112761
PRIMER_LEFT_0_PENALTY=23004.249004
PRIMER_RIGHT_0_PENALTY=28002.863756
PRIMER_LEFT_0_SEQUENCE=TCTCTAGGCTTGGTTCTTGTTC
PRIMER_RIGHT_0_SEQUENCE=GATAAAACTGCCAGAGGGAAAC
PRIMER_LEFT_0=1,22
PRIMER_RIGHT_0=154,22
PRIMER_LEFT_0_TM=57.751
PRIMER_RIGHT_0_TM=59.136
PRIMER_LEFT_0_GC_PERCENT=45.455
PRIMER_RIGHT_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=23000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=28000.000000
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=154
PRIMER_PAIR_0_PRODUCT_TM=73.6
=
SEQUENCE_ID=HMGCR_X9
SEQUENCE_TARGET=0,248
SEQUENCE_TEMPLATE=AAATGATCAGCATGGATATTGAACAAGTTATTACCCTAAGTTTAGCTCTCCTTCTGGCTGTCAAGTACATCTTCTTTGAACAAACAGAGACAGAATCTACACTCTCATTAAAAAACCCTATCACATCTCCTGTAGTGACACAAAAGAAAGTCCCAGACAATTGTTGTAGACGTGAACCTATGCTGGTCAGAAATAACCAGAAATGTGATTCAGTAGAGGAAGAGACAGGGATAAACCGAGAAAGAAAA
PRIMER_LEFT_EXPLAIN=considered 1977, GC content failed 1, low tm 1154, high tm 176, high end compl 1, long poly-x seq 22, ok 623
PRIMER_RIGHT_EXPLAIN=considered 1977, low tm 1125, high tm 203, long poly-x seq 22, ok 627
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=44008.165484
PRIMER_LEFT_0_PENALTY=23004.538081
PRIMER_RIGHT_0_PENALTY=21003.627403
PRIMER_LEFT_0_SEQUENCE=AAATGATCAGCATGGATATTGAA
PRIMER_RIGHT_0_SEQUENCE=TTTTCTTTCTCGGTTTATCCC
PRIMER_LEFT_0=0,23
PRIMER_RIGHT_0=247,21
PRIMER_LEFT_0_TM=58.462
PRIMER_RIGHT_0_TM=57.373
PRIMER_LEFT_0_GC_PERCENT=30.435
PRIMER_RIGHT_0_GC_PERCENT=38.095
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=23000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=21000.000000
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=9.3000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=248
PRIMER_PAIR_0_PRODUCT_TM=73.2
=
SEQUENCE_ID=HMGCR_X10
SEQUENCE_TARGET=0,90
SEQUENCE_TEMPLATE=GTTGAGGTTATAAAACCCTTAGTGGCTGAAACAGATACCCCAAACAGAGCTACATTTGTGGTTGGTAACTCCTCCTTACTCGATACTTCA
PRIMER_LEFT_EXPLAIN=considered 550, low tm 222, high tm 109, ok 219
PRIMER_RIGHT_EXPLAIN=considered 550, low tm 227, high tm 116, ok 207
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=49012.458140
PRIMER_LEFT_0_PENALTY=25006.169796
PRIMER_RIGHT_0_PENALTY=24006.288343
PRIMER_LEFT_0_SEQUENCE=TTGAGGTTATAAAACCCTTAGTGG
PRIMER_RIGHT_0_SEQUENCE=TGAAGTATCGAGTAAGGAGGAGTT
PRIMER_LEFT_0=1,24
PRIMER_RIGHT_0=89,24
PRIMER_LEFT_0_TM=57.830
PRIMER_RIGHT_0_TM=57.712
PRIMER_LEFT_0_GC_PERCENT=37.500
PRIMER_RIGHT_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=25000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=24000.000000
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=89
PRIMER_PAIR_0_PRODUCT_TM=70.2
=
SEQUENCE_ID=HMGCR_X11
SEQUENCE_TARGET=0,89
SEQUENCE_TEMPLATE=TCAGTACTGGTGACACAGGAACCTGAAATTGAACTTCCCAGGGAACCTCGGCCTAATGAAGAATGTCTACAGATACTTGGGAATGCAGA
PRIMER_LEFT_EXPLAIN=considered 540, low tm 135, high tm 249, high end compl 1, ok 155
PRIMER_RIGHT_EXPLAIN=considered 540, low tm 181, high tm 215, ok 144
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=44006.176345
PRIMER_LEFT_0_PENALTY=23002.457721
PRIMER_RIGHT_0_PENALTY=21003.718624
PRIMER_LEFT_0_SEQUENCE=CAGTACTGGTGACACAGGAACC
PRIMER_RIGHT_0_SEQUENCE=TCTGCATTCCCAAGTATCTGT
PRIMER_LEFT_0=1,22
PRIMER_RIGHT_0=88,21
PRIMER_LEFT_0_TM=59.542
PRIMER_RIGHT_0_TM=57.281
PRIMER_LEFT_0_GC_PERCENT=54.545
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=23000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=21000.000000
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=88
PRIMER_PAIR_0_PRODUCT_TM=72.2
=
SEQUENCE_ID=HMGCR_X12
SEQUENCE_TARGET=0,195
SEQUENCE_TEMPLATE=GAAAGGTGCAAAATTCCTTAGTGATGCTGAGATCATCCAGTTAGTCAATGCTAAGCATATCCCAGCCTACAAGTTGGAAACTCTGATGGAAACTCATGAGCGTGGTGTATCTATTCGCCGACAGTTACTTTCCAAGAAGCTTTCAGAACCTTCTTCTCTCCAGTACCTACCTTACAGGGATTATAATTACTCCTT
PRIMER_LEFT_EXPLAIN=considered 1600, low tm 646, high tm 347, ok 607
PRIMER_RIGHT_EXPLAIN=considered 1600, low tm 707, high tm 324, ok 569
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=50013.286753
PRIMER_LEFT_0_PENALTY=23003.785045
PRIMER_RIGHT_0_PENALTY=27009.501708
PRIMER_LEFT_0_SEQUENCE=GAAAGGTGCAAAATTCCTTAGTG
PRIMER_RIGHT_0_SEQUENCE=AAGGAGTAATTATAATCCCTGTAAGGT
PRIMER_LEFT_0=0,23
PRIMER_RIGHT_0=194,27
PRIMER_LEFT_0_TM=59.215
PRIMER_RIGHT_0_TM=57.498
PRIMER_LEFT_0_GC_PERCENT=39.130
PRIMER_RIGHT_0_GC_PERCENT=33.333
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=8.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=23000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=27000.000000
PRIMER_LEFT_0_END_STABILITY=5.7000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=195
PRIMER_PAIR_0_PRODUCT_TM=74.1
=
SEQUENCE_ID=HMGCR_X13
SEQUENCE_TARGET=0,159
SEQUENCE_TEMPLATE=GGTGATGGGAGCTTGTTGTGAGAATGTTATTGGATATATGCCCATCCCTGTTGGAGTGGCAGGACCCCTTTGCTTAGATGAAAAAGAATTTCAGGTTCCAATGGCAACAACAGAAGGTTGTCTTGTGGCCAGCACCAATAGAGGCTGCAGAGCAATAGG
PRIMER_LEFT_EXPLAIN=considered 1240, GC content failed 1, low tm 314, high tm 591, ok 334
PRIMER_RIGHT_EXPLAIN=considered 1240, GC content failed 1, low tm 280, high tm 628, ok 331
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=37006.814000
PRIMER_LEFT_0_PENALTY=18004.525596
PRIMER_RIGHT_0_PENALTY=19002.288404
PRIMER_LEFT_0_SEQUENCE=GGTGATGGGAGCTTGTTG
PRIMER_RIGHT_0_SEQUENCE=CCTATTGCTCTGCAGCCTC
PRIMER_LEFT_0=0,18
PRIMER_RIGHT_0=158,19
PRIMER_LEFT_0_TM=57.474
PRIMER_RIGHT_0_TM=58.712
PRIMER_LEFT_0_GC_PERCENT=55.556
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=18000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=19000.000000
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=159
PRIMER_PAIR_0_PRODUCT_TM=75.5
=
SEQUENCE_ID=HMGCR_X14
SEQUENCE_TARGET=0,158
SEQUENCE_TEMPLATE=TCTTGGTGGAGGTGCCAGCAGCCGAGTCCTTGCAGATGGGATGACTCGTGGCCCAGTTGTGCGTCTTCCACGTGCTTGTGACTCTGCAGAAGTGAAAGCCTGGCTCGAAACATCTGAAGGGTTCGCAGTGATAAAGGAGGCATTTGACAGCACTAGCA
PRIMER_LEFT_EXPLAIN=considered 1230, low tm 83, high tm 898, ok 249
PRIMER_RIGHT_EXPLAIN=considered 1230, low tm 122, high tm 805, ok 303
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=38002.854310
PRIMER_LEFT_0_PENALTY=18002.835611
PRIMER_RIGHT_0_PENALTY=20000.018700
PRIMER_LEFT_0_SEQUENCE=TCTTGGTGGAGGTGCCAG
PRIMER_RIGHT_0_SEQUENCE=TGCTAGTGCTGTCAAATGCC
PRIMER_LEFT_0=0,18
PRIMER_RIGHT_0=157,20
PRIMER_LEFT_0_TM=60.836
PRIMER_RIGHT_0_TM=60.019
PRIMER_LEFT_0_GC_PERCENT=61.111
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=18000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=20000.000000
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=9.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=158
PRIMER_PAIR_0_PRODUCT_TM=78.4
=
SEQUENCE_ID=HMGCR_X15
SEQUENCE_TARGET=0,106
SEQUENCE_TEMPLATE=GATTTGCACGTCTACAGAAACTTCATACAAGTATAGCTGGACGCAACCTTTATATCCGTTTCCAGTCCAGGTCAGGGGATGCCATGGGGATGAACATGATTTCAAA
PRIMER_LEFT_EXPLAIN=considered 710, low tm 257, high tm 250, ok 203
PRIMER_RIGHT_EXPLAIN=considered 710, low tm 172, high tm 343, ok 195
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=44006.746041
PRIMER_LEFT_0_PENALTY=23005.359396
PRIMER_RIGHT_0_PENALTY=21001.386646
PRIMER_LEFT_0_SEQUENCE=GATTTGCACGTCTACAGAAACTT
PRIMER_RIGHT_0_SEQUENCE=TTTGAAATCATGTTCATCCCC
PRIMER_LEFT_0=0,23
PRIMER_RIGHT_0=105,21
PRIMER_LEFT_0_TM=57.641
PRIMER_RIGHT_0_TM=59.613
PRIMER_LEFT_0_GC_PERCENT=39.130
PRIMER_RIGHT_0_GC_PERCENT=38.095
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=7.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=23000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=21000.000000
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=10.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=106
PRIMER_PAIR_0_PRODUCT_TM=72.4
=
SEQUENCE_ID=HMGCR_X16
SEQUENCE_TARGET=0,171
SEQUENCE_TEMPLATE=GGGTACAGAGAAAGCACTTTCAAAACTTCACGAGTATTTCCCTGAAATGCAGATTCTAGCCGTTAGTGGTAACTATTGTACTGACAAGAAACCTGCTGCTATAAATTGGATAGAGGGAAGAGGAAAATCTGTTGTTTGTGAAGCTGTCATTCCAGCCAAGGTTGTCAGAGA
PRIMER_LEFT_EXPLAIN=considered 1360, low tm 684, high tm 181, high end compl 8, high repeat similarity 2, ok 485
PRIMER_RIGHT_EXPLAIN=considered 1360, low tm 616, high tm 248, high end compl 1, ok 495
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=48007.297123
PRIMER_LEFT_0_PENALTY=28005.907183
PRIMER_RIGHT_0_PENALTY=20001.389940
PRIMER_LEFT_0_SEQUENCE=CAGAGAAAGCACTTTCAAAACTT
PRIMER_RIGHT_0_SEQUENCE=TCTCTGACAACCTTGGCTGG
PRIMER_LEFT_0=5,23
PRIMER_RIGHT_0=170,20
PRIMER_LEFT_0_TM=57.093
PRIMER_RIGHT_0_TM=61.390
PRIMER_LEFT_0_GC_PERCENT=34.783
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=28000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=20000.000000
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=166
PRIMER_PAIR_0_PRODUCT_TM=73.1
=
SEQUENCE_ID=HMGCR_X17
SEQUENCE_TARGET=0,141
SEQUENCE_TEMPLATE=AGTATTAAAGACTACCACAGAGGCTATGATTGAGGTCAACATTAACAAGAATTTAGTGGGCTCTGCCATGGCTGGGAGCATAGGAGGCTACAACGCCCATGCAGCAAACATTGTCACCGCCATCTACATTGCCTGTGGACA
PRIMER_LEFT_EXPLAIN=considered 1060, GC content failed 1, low tm 295, high tm 528, high end compl 3, ok 233
PRIMER_RIGHT_EXPLAIN=considered 1060, GC content failed 1, low tm 217, high tm 601, ok 241
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=50007.199789
PRIMER_LEFT_0_PENALTY=29005.780871
PRIMER_RIGHT_0_PENALTY=21001.418918
PRIMER_LEFT_0_SEQUENCE=AAAGACTACCACAGAGGCTATGA
PRIMER_RIGHT_0_SEQUENCE=TGTCCACAGGCAATGTAGATG
PRIMER_LEFT_0=6,23
PRIMER_RIGHT_0=140,21
PRIMER_LEFT_0_TM=57.219
PRIMER_RIGHT_0_TM=59.581
PRIMER_LEFT_0_GC_PERCENT=43.478
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=29000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=21000.000000
PRIMER_LEFT_0_END_STABILITY=5.9000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=135
PRIMER_PAIR_0_PRODUCT_TM=75.5
=
SEQUENCE_ID=HMGCR_X18
SEQUENCE_TARGET=0,159
SEQUENCE_TEMPLATE=GGATGCAGCACAGAATGTTGGTAGTTCAAACTGTATTACTTTAATGGAAGCAAGTGGTCCCACAAATGAAGATTTATATATCAGCTGCACCATGCCATCTATAGAGATAGGAACGGTGGGTGGTGGGACCAACCTACTACCTCAGCAAGCCTGTTTGCA
PRIMER_LEFT_EXPLAIN=considered 1240, GC content failed 18, low tm 522, high tm 395, high repeat similarity 2, ok 303
PRIMER_RIGHT_EXPLAIN=considered 1240, GC content failed 18, low tm 504, high tm 411, ok 307
PRIMER_PAIR_EXPLAIN=considered 62, high end compl 59, ok 3
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=40003.344317
PRIMER_LEFT_0_PENALTY=20000.272067
PRIMER_RIGHT_0_PENALTY=20003.072251
PRIMER_LEFT_0_SEQUENCE=GGATGCAGCACAGAATGTTG
PRIMER_RIGHT_0_SEQUENCE=GCAAACAGGCTTGCTGAGG
PRIMER_LEFT_0=0,20
PRIMER_RIGHT_0=157,19
PRIMER_LEFT_0_TM=60.272
PRIMER_RIGHT_0_TM=62.072
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=57.895
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=20000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=20000.000000
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=158
PRIMER_PAIR_0_PRODUCT_TM=74.5
=
SEQUENCE_ID=HMGCR_X19
SEQUENCE_TARGET=0,155
SEQUENCE_TEMPLATE=GATGCTAGGTGTTCAAGGAGCATGCAAAGATAATCCTGGGGAAAATGCCCGGCAGCTTGCCCGAATTGTGTGTGGGACCGTAATGGCTGGGGAATTGTCACTTATGGCAGCATTGGCAGCAGGACATCTTGTCAAAAGTCACATGATTCACAACA
PRIMER_LEFT_EXPLAIN=considered 1200, low tm 119, high tm 823, ok 258
PRIMER_RIGHT_EXPLAIN=considered 1200, low tm 167, high tm 784, ok 249
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=44006.457265
PRIMER_LEFT_0_PENALTY=21002.049610
PRIMER_RIGHT_0_PENALTY=23004.407655
PRIMER_LEFT_0_SEQUENCE=GATGCTAGGTGTTCAAGGAGC
PRIMER_RIGHT_0_SEQUENCE=TGTTGTGAATCATGTGACTTTTG
PRIMER_LEFT_0=0,21
PRIMER_RIGHT_0=154,23
PRIMER_LEFT_0_TM=58.950
PRIMER_RIGHT_0_TM=58.592
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=34.783
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_0_LIBRARY_MISPRIMING=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=21000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=23000.000000
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=155
PRIMER_PAIR_0_PRODUCT_TM=76.1
=
SEQUENCE_ID=HMGCR_X20
SEQUENCE_TARGET=0,56
SEQUENCE_TEMPLATE=GGTCGAAGATCAATTTACAAGACCTCCAAGGAGCTTGCACCAAGAAGACAGCCTGAATAGCCCGACAGTTCTGAACTGGAACATGGGCATTGGGTTCTAAAGGACTAACATAAAATCTGTGAATTAAAAAAGCTCAATGCATTGTCTTGTGGAGGATGAATAAA
PRIMER_LEFT_EXPLAIN=considered 444, overlap target 99, low tm 91, high tm 152, ok 102
PRIMER_RIGHT_EXPLAIN=considered 1150, GC content failed 42, low tm 311, high tm 512, high any compl 26, high end compl 3, long poly-x seq 20, ok 236
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=24265.616387
PRIMER_LEFT_0_PENALTY=24004.237346
PRIMER_RIGHT_0_PENALTY=261.379041
PRIMER_LEFT_0_SEQUENCE=GGTCGAAGATCAATTTACAAGACC
PRIMER_RIGHT_0_SEQUENCE=GCCCATGTTCCAGTTCAGA
PRIMER_LEFT_0=0,24
PRIMER_RIGHT_0=87,19
PRIMER_LEFT_0_TM=60.237
PRIMER_RIGHT_0_TM=59.621
PRIMER_LEFT_0_GC_PERCENT=41.667
PRIMER_RIGHT_0_GC_PERCENT=52.632
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_PAIR_0_LIBRARY_MISPRIMING=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_0_POSITION_PENALTY=24000.000000
PRIMER_RIGHT_0_POSITION_PENALTY=260.000000
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=88
PRIMER_PAIR_0_PRODUCT_TM=73.6
=
