PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 1 any right primer - OK

No mispriming library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          1   20   51.06   30.00 11.00  5.00 cttgattcaatgaatctcaa
RIGHT PRIMER        65   18   48.54   27.78  2.00  2.00 aaaaaaacacaatccAga
SEQUENCE SIZE: 100
INCLUDED REGION SIZE: 100

PRODUCT SIZE: 65, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: 1,20,,

    1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt
      >>>>>>>>>>>>>>>>>>>>                           <<<<<<<<<<<<<

   61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
      <<<<<                                   

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
primer3 release 2.2.0


PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 2 any left primer - OK

No mispriming library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          1   20   51.06   30.00 11.00  5.00 cttgattcaatgaatctcaa
RIGHT PRIMER        65   17   45.95   29.41  2.00  1.00 aaaaaaacacaatccAg
SEQUENCE SIZE: 100
INCLUDED REGION SIZE: 100

PRODUCT SIZE: 65, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: ,,49,17

    1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt
      >>>>>>>>>>>>>>>>>>>>                            <<<<<<<<<<<<

   61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
      <<<<<                                   

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
primer3 release 2.2.0


PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 3 any pair - OK

No mispriming library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          1   20   51.06   30.00 11.00  5.00 cttgattcaatgaatctcaa
RIGHT PRIMER        65   16   43.92   25.00  2.00  0.00 aaaaaaacacaatccA
SEQUENCE SIZE: 100
INCLUDED REGION SIZE: 100

PRODUCT SIZE: 65, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: ,,,

    1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt
      >>>>>>>>>>>>>>>>>>>>                             <<<<<<<<<<<

   61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
      <<<<<                                   

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
primer3 release 2.2.0


PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 4 constrained left and right primers - OK

No mispriming library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          1   20   51.06   30.00 11.00  5.00 cttgattcaatgaatctcaa
RIGHT PRIMER        65   17   45.95   29.41  2.00  1.00 aaaaaaacacaatccAg
SEQUENCE SIZE: 100
INCLUDED REGION SIZE: 100

PRODUCT SIZE: 65, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: 1,20,49,17

    1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt
      >>>>>>>>>>>>>>>>>>>>                            <<<<<<<<<<<<

   61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
      <<<<<                                   

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
primer3 release 2.2.0


PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 5 constrained left and right primers - NOT OK

No mispriming library specified
Using 1-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 100
INCLUDED REGION SIZE: 100

PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: 5,10,60,10

    1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt
   61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     1     0     0     0     0     0     0     0     0     0
Right      1     0     0     0     1     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
primer3 release 2.2.0


PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 6 constrained left and right primers - OK

No mispriming library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          1   20   51.06   30.00 11.00  5.00 cttgattcaatgaatctcaa
RIGHT PRIMER        65   17   45.95   29.41  2.00  1.00 aaaaaaacacaatccAg
SEQUENCE SIZE: 100
INCLUDED REGION SIZE: 100

PRODUCT SIZE: 65, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: 5,10,60,7 1,25,,

    1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt
      >>>>>>>>>>>>>>>>>>>>                            <<<<<<<<<<<<

   61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
      <<<<<                                   

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
primer3 release 2.2.0


PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 7 contrained left and right primers - NOT OK

No mispriming library specified
Using 1-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 100
INCLUDED REGION SIZE: 100

PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: 1,19,49,17 1,20,50,16

    1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt
   61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, not in any ok region 1, ok 0
primer3 release 2.2.0


PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 8 any left primer - NOT OK

No mispriming library specified
Using 1-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 100
INCLUDED REGION SIZE: 100

PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: ,,49,16

    1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt
   61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     1     0     0     0     0     0     0     0     0     0
Pair Stats:
considered 0, ok 0
primer3 release 2.2.0


PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 9 any right primer - NOT OK

No mispriming library specified
Using 1-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 100
INCLUDED REGION SIZE: 100

PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: 2,20,,

    1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt
   61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     1     0     0     0     0     0     0     0     0     0
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 0, ok 0
primer3 release 2.2.0


PRIMER PICKING RESULTS FOR SEQUENCE_PRIMER_PAIR_OK_REGION_LIST test 10 any right primer, any left primer - OK

No mispriming library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER          1   20   51.06   30.00 11.00  5.00 cttgattcaatgaatctcaa
RIGHT PRIMER        65   17   45.95   29.41  2.00  1.00 aaaaaaacacaatccAg
SEQUENCE SIZE: 100
INCLUDED REGION SIZE: 100

PRODUCT SIZE: 65, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
PAIR_OK_REGIONS (left_start, left_len, right_start, right_len)*: 2,20,, ,,49,17

    1 cttgattcaatgaatctcaaaaatggattactattgttcattctgtttcTggattgtgtt
      >>>>>>>>>>>>>>>>>>>>                            <<<<<<<<<<<<

   61 tttttcaaagttgaatccaaatgtgtaaaagggtgtgatg
      <<<<<                                   

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl     X  stab    ok
Left       1     0     0     0     0     0     0     0     0     0     0     0     0     1
Right      1     0     0     0     0     0     0     0     0     0     0     0     0     1
Pair Stats:
considered 1, ok 1
primer3 release 2.2.0


